Triphenyltin Exposure Causes Irreversible Inhibition of the Cytotoxic Function of Human Natural Killer Cells
Description
Previously, we have shown that exposure to TPT (1 µM for 1 h) greatly reduced the ability of NK cells (obtained from peripheral blood of adult male and female volunteer donors) to kill K562 (human chronic myelogenous leukemia) tumor cells [12]. The ability of NK cells to lyse tumor targets was measured using a 51Cr release assay [12]. In this study we examined whether the inhibition of NK-cell cytotoxicity induced by a brief (1 h) exposure to TPT could be reversed if the TPT was removed and the cells were allowed to incubate for up to 6 days in TPT-free media.
|