The Thermo Scientific™ Orbitrap Exploris™ 240 mass spectrometer is a Thermo Scientific™ quadrupole-Orbitrap™ mass spectrometer, which is based on proven hardware and instrument control software designs of the next-generation Thermo Scientific mass spectrometers.1The Orbitrap Exploris 240 mass spectrometer provides the performance and versatility needed to drive discovery and identification, and the quantitative precision and accuracy to confidently scale up for impact. Operational simplicity with intelligent acquisition of high-resolution accurate-mass data (HRAM) provides the fast path to high-quality results for users of all skill levels in a range of applications including small molecule, peptide, and intact protein analyses.
Benefits
- Premium quantitative and qualitative performance with the fast-scanning High-Field Thermo Scientific™ Orbitrap™ mass analyzer
- Compatible with Thermo Scientific next-generation ion sources including the Thermo Scientific™FAIMS Pro™ interface
- Comprehensive, automated sample profiling with Thermo Scientific™AcquireX™ data acquisition workflow
- Set up and forget with at least five days of maximum mass accuracy provided, using built-in Thermo Scientific™ EASY-IC™ ion source internal reference mass
- Extended mass transmission and detection up to m/z 8,000 with the Thermo Scientific™ BioPharma option
Hardware features
Ion source
Thermo Scientific™ OptaMax™ NG ion source
- Adjustable heated electrospray ionization (H-ESI II) probe for ultimate sensitivity
- Additional non-heated low-flow calibrant sprayer for optimal mass and system calibration
- Designed for maximum performance with minimum adjustment
- Enhanced ruggedness and reduced chemical noise with sweep gas
- Flow rates from 1 μL/min to 1,000 μL/min; OPTON-30139 (low-flow needle; 1–10 μL/min), OPTON-30694 (high-flow needle; 5–1,000 μL/min)
Ion optics
The atmospheric pressure ionization (API) interface consists of:Round bore transfer tube
- Removable, heated ion transfer tube
- Vent-free maintenance
S-lens
S-lens stacked-ring radio frequency (RF) ion guide captures and efficiently focuses the ions into a tight beam. Large variable spacing between electrodes allows for better pumping efficiency and improved ruggedness.
Advanced active beam guide (AABG)
Axial field and injection filtering reduce noise by preventingneutrals and high-velocity clusters from entering the quadrupole mass filter using double bent design geometry
Advanced quadrupole technology (AQT)
- Segmented quadrupole mass filter for precursor ion selection with variable precursor isolation width from 0.4 to 1,200 Da
- MS/MS precursor ion selection with high transmission from m/z 40 to 2,500
Ion-routing multipole (IRM)
- Robust ion trapping for MS scans and higher energy collisional dissociation (HCD)
- Selection of absolute collision energy (CE) or normalizedcollision energy (NCE) for precursor fragmentation
- Nitrogen collision gas
Automatic gain control (AGC)
Reliable AGC measurements for controlled injection of the number of ions is ensured by the novel Independent Charge Detector
Thermo Scientific Orbitrap mass analyzer
- High-Field Orbitrap mass analyzer
- Low noise detection pre-amplifier
- 4kV central electrode voltage
Vacuum system
- A compact single turbo pump design providing the adequate vacuum in six stages for the aluminum high-vacuum analyzer chambers
- Advanced vacuum technology reduces pressure in the ultra-high vacuum regions, enhancing transmission of ions to the Orbitrap mass analyzer
Thermo Scientific EASY-IC ion source
- Provides <1 ppm RMS mass accuracy under defined conditions with minimum effort for at least five days
- Generates internal calibrant ions for real-time mass calibration of spectra in both positive and negative modes
Optional hardware
BioPharma option
- Analysis of large molecules including intact proteins and large complexes sprayed under denatured or native conditions
- Includes mass transmission and detection up to m/z 8,000
Thermo Scientific™ Nanospray Flex™ NG ion source
- Supports static and dynamic electrospray experiments
- Compatible with liquid flow rates of 50 nL/min to 2 μL/min
APCI probe for OptaMax NG ion source
- Compatible with flow rates from 50 μL/min to 1,000 μL/minwithout splitting
- The APCI probe can be upgraded to APPI
Thermo Scientific FAIMS Pro interface
- FAIMS Pro interface (high field asymmetric ion mobility spectrometry) augments analytical selectivity, enabling identification and quantitation of more proteins while reducing time-consuming sample preparation
- Performs online gas-phase fractionation based on differential ion mobility
- Optimized for 100–1,000 nL/min flow rates
