EBI PRIDE Archive is a shotgun proteomics mass spectrometry database that includes shotgun proteomics-related mass spectrometry data, protein identification, post-translational modification, and other mass spectrometry identification information. It is able to provide relevant reference for shotgun proteomics research.
Expasy is the SIB Swiss Institute of Bioinformatics registry of bioinformatics software tools, database/knowledgebase resources, and data sets developed by SIB members. It provides detailed information about software tools, databases and data sets that support a range of life science and clinical research domains, from genomics, proteomics and structural biology, to evolution and phylogeny, systems biology and medical chemistry.
It is a large, continuously updated database containing many massive spectrometric data for proteins identified by mass spectrometry. GPMdb is very simple. You can obtain the mass spectrometry identification data of the protein, including peptide sequences, post-translational modification and so on, just by searching the protein name, gene name, motif and corresponding species. This database can provide direct mass spectral data reference, and is quite practical.
The Universal Protein Resource (UniProt) is a comprehensive resource for protein sequence and annotation data. The UniProt databases are the UniProt Knowledgebase (UniProtKB), the UniProt Reference Clusters (UniRef), and the UniProt Archive (UniParc). UniProt is a collaboration between the European Bioinformatics Institute (EMBL-EBI), the SIB Swiss Institute of Bioinformatics and the Protein Information Resource (PIR). Across the three institutes more than 100 people are involved through different tasks such as database curation, software development and support. EMBL-EBI and SIB together used to produce Swiss-Prot and TrEMBL, while PIR produced the Protein Sequence Database (PIR-PSD). These two data sets coexisted with different protein sequence coverage and annotation priorities.
The peptide calculator is a convenient tool for scientists as a molecular weight peptide calculator, which can be used as an amino acid calculator as well. Additionally, the tool includes a hydrophobicity calculator, a net charge calculator at different pH, isoelectric point calculator and the hydrophilicity ratio.
Similarly to different oligonucleotide calculators that can be found on the internet, the BACHEM Oligo calculator will provide the molecular weight of the desired oligonucleotide with or without a DMT group and 2’-O modifications.
The Toolbox contains a set of mass spectrometry-related utility programs which provide for calculation of oligonucleotide mass values for RNA and DNA and enzymatic cleavage fragments, and of sequence ions from collision-induced dissociation, all with provision for use of modified residues.
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