|
Instrument Database:
Gilson, Inc - 925 PC Workstation
| |
|
|
| |
|
| |
|
| Year of introduction |
|
| Status |
available |
| Company |
Gilson, Inc
|
| Categories |
Automation: Workstation
|
The 925 PC Workstation automates the preparation of high-throughput protein crystallography. It is engineered to meet the demand for high-throughput reagent screening, yet can provide large, high-quality crystals suitable for X-ray diffraction.
- Processes up to four sitting drop plates per batch
- Significantly reduces the time and expense needed to determine strategies for optimization experiments—processes 96 samples in less than 7 minutes
- Flexibility of using time-tested hanging or sitting drop vapor diffusion techniques—no need to validate new methods
- Multitool head allows for plate gripper and 96- and eight-channel pipettors for fast transfer of screening and protein solutions
- Eliminates carryover and cross-contamination by using disposable pipette tips for accurate, repeatable and dependable results (0.5 µL of 30% PEG 8000 at <3% CV)
- Deck layout holds plates, tips, screening solutions and protein reservoirs on the same bed, allowing for complete and quick automation
- Aspirates protein in volumes as low as 0.5 µL, which saves precious protein sample and costly reagents
Applications
- High-throughput crystallography rapid screening protocols
- Rapid and efficient screening of large numbers of different proteins
|
| Specifications |
|
|
Liquid Handling
Reproducibility: <3% CV with 0.5 µL of 30% PEG 8000
Accuracy: 101% with 0.5 µL of 30% PEG 8000
Arm Speed (max.)
X/Y: 152.4 cm/sec (60 in./sec)
Z: 25.4 cm/sec (10 in./sec)
Compressed Air Requirements
80 psi (5.3 bar) @ 2 cfm
Environmental Conditions
Indoor use
Altitude: up to 2000 m
Temperature range: 5–40°C
Air pressure: 75–105 kPa
Pollution degree: 1 or 2, in accordance with IEC 66
Humidity: Maximum relative humidity 80% for temperatures up to 31°C, decreasing linearly to 50% relative humidity at 40°C
Maximum Syringe Flow Rates for Water
1000 µL/sec; adjustable from 75 to 1000 µL/sec
Manufacturing Standards
Meets applicable Safety and EMC certification standards; UL and CE certified
Power Requirements
Frequency: 50–60 Hz
Voltage: 100–120 or 220–240V; mains voltage fluctuations not to exceed ±10% of the nominal voltage
Current rating: 10A for 115V AC (20A with chiller) or 7A for 230V AC (15A with chiller)
Probe Positioning Performance
Accuracy: ±0.2 mm (0.008 in.) in X/Y/Z dimensions
Repeatability: ±0.1 mm (0.004 in.) in X/Y/Z dimensions
Sampler Type
Gantry style X/Y/Z with stationary rack design
Software
Via Gilson 745 Control Software
Syringe Capacity (maximum)
96-channel: 200 µL
8-channel: 50 µL
Single-channel: 200 µL
Syringe Pump
Internal, high-precision, piston
Temperature Range
5–40°C*
*Actual temperature is a function of the ambient temperature and the style of microplates used.
Typical Number of Experimental Drops per Hour
1110+
Typical Volume of Protein Required
100 µL (about 10 µL per 8 sample vials)
Typical Volume of Screening Solution Required
50 µL per 96 wells
Vacuum Requirements
25” Hg @ 2 cfm
Vertical Punch Strength
96-Well: 4.5 kg (10 lbs.)
Gripper: 8.2 kg (18 lbs.)
Dimensions* (w x d x h) *Include air gap and connectors
122 x 96 x 88 cm (48 x 37.8 x 34.6 in.)
Weight
132 kg (291 lbs.)
|
|
