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Instrument Database:
Agilent Technologies Inc. - G1377A (1200 Series) Micro Well-Plate Autosampler
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| Year of introduction |
2006 |
| Status |
available |
| Company |
Agilent Technologies Inc.
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| Categories |
Automation: Autosampler (LC)
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For capillary and nano LC
The Agilent 1200 Series micro well-plate autosampler is designed to perform capillary and nano LC, allows injection of sample volumes from nL to µL ranges from well plates and vials. It can be upgraded from non-thermostatted to thermostatted version by adding the Agilent 1200 Series Thermostat for ALS/FC/spotter.
Features:
- Minimized dispersion using a micro Rheodyne® valve and the optimized design of the needle seat, loop and seat capillaries minimize dispersion.
- Ten times better resolution than a standard autosampler by using a high-resolution metering device.
- Minimal delay volumes for rapid gradients and fast equilibration when bypassing the autosampler after sample injection.
- Increased sample injection speed for high sample throughput.
- No waste of sample (especially when available sample is limited) through variable injection volume and flow through design (compared to fixed loop approaches).
- Flexible and convenient sample handling with different types of sample containers such as well-plates and vials. Using 384-well plates allows you to process up to 768 samples unattended.
- Peltier temperature control from 4 to 40 °C for thermally-labile samples (upgrade from non-thermostatted to thermostatted version possible).
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| Specifications |
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| Performance Specifications 1200 Series Micro Well-Plate Autosampler | | Injection range | 0.01 - 8 µL in 0.01 µL increments with the small loop capillary
0.01 - 40 µL in 0.01 µL increments with the extended loop capillary | | Precision | Typically < 0.5 % RSD of peak areas from 5 - 40 µL,
Typically < 1 % RSD from 1 - 5 µL
Typically < 3 % RSD from 0.2 - 1 µL
Variable volume | | Sample viscosity range | 0.2 - 5 cp | | Sample capacity | 2 well plates (96 and 384) plus 10 additional 2-mL vials
108 x 2-mL vials in 2 x 54 vial plate plus 10 additional 2-mL vials
30 x 6-mL vials in 2 x 15 vial plate plus 10 additional 2-mL vials
54 Eppendorf tubes (0.5/1.5/2.0mL) in 2 x 27 Eppendorf tube plate
Also compatible with the Agilent 1200 Series sample capacity extension for further expansion of the sample capacity | | Injection cycle time | Typically < 30 s using following standard conditions:
Default draw speed: 4 µL/min
Default eject speed: 10 µL/min
Injection volume: 0.1 µL | | Carry-over | Typically < 0.05 % using the following conditions:
Column: 150 x 0.5 mm Hypersil ODS, 3 µm
Mobile phase: Water/Acetonitrile = 85/15
Column Flow rate: 13 µL/min
Injection volume: 1 µL caffeine (=25ng caffeine), 1 µL water to test carryover
Outside wash of needle before injection: 20 sec with water using flush port |
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