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Instrument Database:
Dionex Corporation - SFM Dual-Arm Sample and Fraction Manager
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| Year of introduction |
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| Status |
historical ( out of sale ) |
| Company |
Dionex Corporation
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| Categories |
Automation: Autosampler (LC)
Automation: Fraction Collector (LC)
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The Sample and Fraction Manager (SFM TM ) is a combined autosampler and fraction collector with unmatched capacity and versatility. Its unique dual-arm design allows analytical and preparative scale injections with dedicated injection syringes, valves, and flow paths. The SFM is capable of collecting fractions and preparing the next injection (including wash cycles) in parallel. This operation mode reduces the overall cycle time and increases the throughput on your purification system. |  | Overlapping Sample Preparation
Shorten cycle times preparing the next injection while collecting fractions from the current run. Overlapping sample preparation is possible with the SFM. The instrument has two independently movable arms, the right arm for preparative injections and the left for fraction collection and analytical injections. This design allows your SFM sufficient time to wash the injection needle and injection valve, and prepare the next injection without extending the cycle time. The right arm performs these operations while the left arm continues to collect fractions. The result is short cycle times and zero sample carryover. | | Tandem Operation
The SFM features two completely separated flow paths. Thanks to the dual-arm design, dedicated valves, and syringes, even high-concentrated samples can never contaminate low-concentrated fractions. Additionally, while performing fraction collection and sample preparation, the SFM can carry out as many wash cycles as needed without extending your run times. The dual-wash station also facilitates rinsing with different solvents. | | Zero Sample or Fraction Carryover
The SFM features two completely separated flow paths. Thanks to the dual-arm design, dedicated valves, and syringes, even high-concentrated samples can never contaminate low-concentrated fractions. Additionally, while performing fraction collection and sample preparation, the SFM can carry out as many wash cycles as needed without extending your run times. The dual-wash station also facilitates rinsing with different solvents. A unique syringe valve with an extremely small dead volume between the valve and the collection outlet further prevents fraction carryover.
| | Unmatched Capacity
The SFM features a class-leading capacity with six tray positions on the injection side, for well plates, deep well plates, 2- and 4-mL vials, and 14 fraction racks on the collection side for 1092 9-mL or 294 50-mL tubes. With this capacity, you can enjoy your weekend while the SFM continues processing one sample after the other. | | Powerful Control by Chromeleon Software
Chromeleon® software provides powerful bidirectional control for the SFM. The software manages overlapping sample preparation, documents all instrument settings in audit trails, and monitors System Wellness parameters. Clear injection tray and fraction rack views, and powerful tracking functions are part of the Chromeleon Purification Suite TM . | | Tandem Operation
Shorten you cycle times and increase your sample throughput by equipping the SFM with the Tandem Operation Kit. In tandem mode, cycle times are shortened by equilibrating one column while the other one is used simultaneously for sample purification. As soon as the purification run is completed, the columns are switched and the next preparative sample can immediately be injected. |
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| Specifications |
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| Sample and Fraction Manager (SFM) capabilities | You can wash the preparative injection syringe and valve thoroughly, and prepare the next injection while collecting fractions—all without any delay of the next injection | | SFM injection volume range, prep. arm | 1–5000 μL | | SFM injection volume range, analytical arm (Analytical Inj. Kit required) | 0.1–80 μL | | Sample Capacity | Provides six positions for deep well plates,2-mL vial racks or 4-mL vial racks - With 6 deep-well plates: 576 samples
- With 6 2-mL vial racks: 324 samples
- With 6 4-mL vial racks: 144 samples
| | Fraction Capacity | The SFM provides 14 rack positions and the following total capacities: - 78-position racks for 9-mL tubes,
(13 × 100 mm): 1092 fractions - 50-position racks for 12-mL tubes,
(16 ×100 mm): 700 fractions - 50-position racks for 20-mL tubes,
(16 × 150 mm): 700 fractions - 36-position racks for 27-mL tubes,
(18 × 150 mm): 504 fractions - 36-position racks for 32-mL tubes,
(20 × 150 mm): 504 fractions - 21-position racks for 50-mL tubes,
(25 × 150 mm): 294 fractions - 18-position racks for 50-mL Falcon
tubes (29 × 116 mm): 252 fractions
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