nanoLC-1Dplus system specifications system components
- Binary gradient pump
- Dedicated sample loading pump
- 10-port, low-volume, biocompatible injection valve (Ti or PAEK)
- Optional temperature-controlled autosampler (96- or 384-well plate compatible) with a 6-port biocompatible sample injection valve (Ti or PAEK)
flow rate range
20-1000 nL/min (other ranges available with flow module swap) 1-30 µL/min for sample loading pump
pump type
Microfluidic direct pumping system with independent flow control feedback for each mobile phase. Retention time RSD < 0.5% at 500 nL/min
gradient formation
High-pressure gradient mixing, no flow splitting
delay volume
65 nL from mixer to column
delay time
< 25 sec at 500 nL/min
mobile phase compatibility
All mobile phases compatible with 316 stainless steel, PEEK, and silica
injections
Standard, metered and rapid injection modes
injection volume
200-50 µL; option for larger sample volumes available
columns
Optimized for use with 50-150 µm i.d. columns, 5-15 cm in length
peak parking
Response time from 500-100 nL/min typically < 4 seconds at spray tip. Response time can vary with external hardware configuration
autosampler
One 96- or 384-well plate capacity Peltier type temperature maintained sample compartment. 5-40° C; cooling capacity 12° C below ambient Dual needle design for seal piercing Integral 6-port biocompatible injection valve
system control
Computer with graphical user interface for control of all system parameters. Software allows import of run tables and creates CDF, text, and Excel files for data export and analysis. Tracking of instrument runtime, column usage, total injections, solvent usage, and error codes. System drivers available for Thermo Electron’s Xcalibur™, Applied Biosystems/MDS SCIEX Analyst®, and Bruker Daltonics’ HyStar™ mass spectrometer software.
power requirements
100-240 VAC, 4/63 Hz, 3A
dimensions
- Width: 14 inches (35 cm)
- Depth: 15 inches (38 cm)
- Height: 8 inches (20 cm)
- Computer: additional lab spaced needed for computer, keyboard, mouse, and monitor
