Tired of uncertainties and unpleasant surprises on Monday mornings? Summit System Wellness technology saves time and cost by ensuring high uptimes and no unpleasant surprises. Instrument self-tests and System Wellness checks guarantee maximum reliability and continuous optimal operation. Unlike other systems that provide only basic parameter counters, Summit’s System Wellness technology actually monitors the status of the most crucial variables, such as the intensity of the detector lamp or tightness of the piston seals (patented). System Wellness informs you about small problems before they become big problems, allowing you to plan ahead for maintenance for minimum impact on your day-to-day work schedule.
Summit HPLC technology allows for shorter analysis times, and therefore higher sample throughput than conventional HPLC systems. These advantages are made possible by superior pump performance, smaller gradient delay volumes, faster injector cycle times, and the suitability of the system for high-temperature operation. With Summit HPLC, you can speed up your HPLC separations by using shorter and smaller particle columns, faster and steeper gradients, shorter equilibration times, and higher temperatures.
The advanced design of the Summit HPLC system ensures accurate and precise results, lowest detection limits, and superior separation power. Dionex SmartFlow TM pump technology enables Summit pumps to deliver precise, accurate, and pulse-free gradients without the need for a pulse damper. Some manufactures still rely on pulse dampers, which increase gradient delay volume and dispersion, and have a detrimental effect on retention time reproducibility when column backpressure increases. The Summit HPLC system provides superior separations at elevated temperatures, because eluents are preheated before entering the column. This preheating ensures even temperatures within the column and sharp, well-separated peaks.
All modules provide optimal performance for your application needs, from sensitive microflow LC/MS analyses to automated semipreparative purifications. The P680 pump system uses a variable-volume mixing chamber for lowest gradient delay when delivering microflows, and for complete mixing when using poorly miscible eluents. The ASI-100TM Autosampler supports 8 different vial formats, including, for example, Eppendorf test tubes. Support for different vial formats often eliminates the need for sample transfer to specific autosampler vials. The TCC-100 Thermostatted Column Compartment guarantees stable retention times in a large temperature range, from 5 to 85 °C.
Maintaining regulatory compliance can significantly add to the laboratory workload. With the Summit system, achieving compliance is fast and straightforward. The AutoQ™ equipment qualification routines (standard with Chromeleon software) allow fully automated Installation Qualification (IQ), Operational Qualification (OQ), and Performance Qualification (PQ). The tests take only about 30 min of the operator’s time for setup, and then run unattended, performing the tests and generating a comprehensive report for another 4 to 5 h. Compared with common qualification procedures that can occupy a day or more of a qualified specialist’s time, AutoQ equipment qualification saves a considerable amount of time and money. Furthermore, Summit HPLC provides complete documentation of all relevant data, including analysis parameters, as well as warning and error messages in audit trails. A column identification system ensures automated documentation of column variables, such as the number of injections on a column, the maximum pressure it has been exposed to, or its expiration date.
Isocratic: 1–10,000 µL/minBinary HPG: 1–10,000 µL/minQuaternary LPG: 1–10,000 µL/minSemipreparative: 0.2–50 mL/min
Analytical versions: ±0.1% at 1 mL/minSemipreparative: ±1.0% at 2 mL/min
Binary HPG: <150 µLQuaternary LPG: <400 µLSemipreparative: <150 µL
Binary HPG: <70 µLQuaternary LPG: <300 µL
Extension 1: 600 µLExtension 2: 1250 µL
Quaternary LPG: built-in; 4 channelsOther versions: external (optional)
Micro: 192 1.2-mL vialsAnalytical: 117 2-mL vialsSemipreparative: 63 4-mL vials
0.1–250 µL
Micro: 0.1–100 µLSemipreparative: 1–1000 µLSemipreparative: 1–2500 µL
5–100 µL injections: typically <0.2% RSD1–5 µL injections: typically <1% RSD
Temperature range: Settable from 4–45 °C
Temperature range: 5–85 °CTemperature accuracy: ±0.5 °CTemperature stability: ±0.1 °CTemperature precision: ±0.1 °CCooling/Heating performance: Less than 20 min from 20 to 50 °C
Wavelength range: 200–595 nmNoise: ±5 µAU, 254 nmSignals: Up to 4 wavelengthsThe detector is upgradeable to the photodiode array version UVD 340U
Wavelength range: 200–595 nmNoise: ±5 µAU, 254 nmSpectral resolution: 1.9 nm (UV); 3.3 nm (Vis)
Wavelength range: 190–800 nmNoise: ±10 µAU, 254 nmSpectral resolution: 1 nmLamps: Deuterium and tungsten
Excitation wavelength range: 200–650 nmEmission wavelength range: 200–650 nmSensitivity: S/N ratio of >300 for the Raman line of waterWavelength Scanning: For excitation and emission wavelengths
Mass range: 50–2000 m/z (standard); 18–1500 m/z (enhanced low-mass option)Sensitivity, Positive ion ESI (5 pg erythromycin inj.): 100:1 s/n ratioSensitivity, Negative ion ESI (2 pg p-nitrophenolinj.): 50:1 s/n ratioSensitivity, Positive ion APCI (50 pg erythromycin inj.): 200:1 s/n ratioSensitivity, Negative ion APCI (20 pg p-nitrophenol inj.): 50:1 s/n ratio
