With a Summit® HPLC system, you benefit from both modularity and a high level of system integration. Combine high-performance isocratic and gradient pumps with the injectors, column compartments, and detectors that best satisfy your needs. Enjoy the advantages of an integrated system, with optimized flow paths, seamless intermodule coordination, and powerful Chromeleon® software.
Tired of uncertainties and unpleasant surprises on Monday mornings? Summit System Wellness technology saves time and cost by ensuring high uptimes and no unpleasant surprises. Instrument self-tests and System Wellness checks guarantee maximum reliability and continuous optimal operation. Unlike other systems that provide only basic parameter counters, Summit’s System Wellness technology actually monitors the status of the most crucial variables, such as the intensity of the detector lamp or tightness of the piston seals (patented). System Wellness informs you about small problems before they become big problems, allowing you to plan ahead for maintenance for minimum impact on your day-to-day work schedule.
High Sample Throughput
Summit HPLC technology allows for shorter analysis times, and therefore higher sample throughput than conventional HPLC systems. These advantages are made possible by superior pump performance, smaller gradient delay volumes, faster injector cycle times, and the suitability of the system for high-temperature operation. With Summit HPLC, you can speed up your HPLC separations by using shorter and smaller particle columns, faster and steeper gradients, shorter equilibration times, and higher temperatures.
The advanced design of the Summit HPLC system ensures accurate and precise results, lowest detection limits, and superior separation power. Dionex SmartFlow TM pump technology enables Summit pumps to deliver precise, accurate, and pulse-free gradients without the need for a pulse damper. Some manufactures still rely on pulse dampers, which increase gradient delay volume and dispersion, and have a detrimental effect on retention time reproducibility when column backpressure increases. The Summit HPLC system provides superior separations at elevated temperatures, because eluents are preheated before entering the column. This preheating ensures even temperatures within the column and sharp, well-separated peaks.
All modules provide optimal performance for your application needs, from sensitive microflow LC/MS analyses to automated semipreparative purifications. The P680 pump system uses a variable-volume mixing chamber for lowest gradient delay when delivering microflows, and for complete mixing when using poorly miscible eluents. The ASI-100TM Autosampler supports 8 different vial formats, including, for example, Eppendorf test tubes. Support for different vial formats often eliminates the need for sample transfer to specific autosampler vials. The TCC-100 Thermostatted Column Compartment guarantees stable retention times in a large temperature range, from 5 to 85 °C.
Regulatory Compliance Without the Pain
Maintaining regulatory compliance can significantly add to the laboratory workload. With the Summit system, achieving compliance is fast and straightforward. The AutoQ™ equipment qualification routines (standard with Chromeleon software) allow fully automated Installation Qualification (IQ), Operational Qualification (OQ), and Performance Qualification (PQ). The tests take only about 30 min of the operator’s time for setup, and then run unattended, performing the tests and generating a comprehensive report for another 4 to 5 h. Compared with common qualification procedures that can occupy a day or more of a qualified specialist’s time, AutoQ equipment qualification saves a considerable amount of time and money. Furthermore, Summit HPLC provides complete documentation of all relevant data, including analysis parameters, as well as warning and error messages in audit trails. A column identification system ensures automated documentation of column variables, such as the number of injections on a column, the maximum pressure it has been exposed to, or its expiration date.
Excitation wavelength range: 200–650 nm Emission wavelength range: 200–650 nm Sensitivity: S/N ratio of >300 for the Raman line of water Wavelength Scanning: For excitation and emission wavelengths
MSQ™ Mass Spectrometric Detector
Mass range: 50–2000 m/z (standard); 18–1500 m/z (enhanced low-mass option) Sensitivity, Positive ion ESI (5 pg erythromycin inj.): 100:1 s/n ratio Sensitivity, Negative ion ESI (2 pg p-nitrophenolinj.): 50:1 s/n ratio Sensitivity, Positive ion APCI (50 pg erythromycin inj.): 200:1 s/n ratio Sensitivity, Negative ion APCI (20 pg p-nitrophenol inj.): 50:1 s/n ratio