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Instrument Database:
Dionex Corporation - WPS-3000 Well Plate Sampler
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| Year of introduction |
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| Status |
available |
| Company |
Dionex Corporation
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| Categories |
Automation: Autosampler (LC)
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Features
- Three well plate capacity (up to 1152 samples)
- Integrated sample thermostatting, 4–45 °C (patent pending)
- Sealed sample compartment for outstanding temperature stability
- Allows injections of thermo labile proteins
- Dual-needle injection principle
- Large injection range: 20 nL to 100 µL
- Unmatched injection reproducibility
- Lowest carry-over: 0.01%
The WPS-3000 Well Plate Sampler has been specifically designed to provide unattended sample throughput, combined with superior reproducible injections, of even smallest samples volumes.
The sample rack handles up to three well plates, 96 or 384 wells—sealed or unsealed—or sample vial trays, or combinations thereof. The maximum total capacity of the system is 1152 samples. Flawlessly exchange sample plates or use priority samples during a run sequence using the Well Plate Sampler.
Applying the cooling option, the sample rack is completely sealed, opening only during the injection cycle. This generates optimal temperature stability throughout the thermostatting range of 4–45 °C. This stability guarantees optimal storage of thermo-labile samples.
Using the dual-needle principle, the vial septum ot the well seal is pierced by the stainless steel outer needle. The sampler guides the sampling needle, made of a fused silica capillary, through the piercing needle down to the bottom of the sample carrier. Easily program the sample needle height to match sample vial or well deepness. Injections of minute sample volumes, with virtually zero sample loss—1 µL injection per 1 µL sample—are performed using the µL-pickup routine. This routine is of particular importance when injecting protein samples that cannot be diluted without the risk of protein loss or alteration.
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| Specifications |
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Sample Capacity
3× well plate (128 × 86 mm); 15× 10-mL vials for reagents, diluents, and transport liquids
Sample Format
96 well plate, 96 deep well plate, 384 well plate, 40 standard autosampler vials 1.5 mL (sealed or open)
Injection Volume
20 nL to 25 µL with 25-µL syringe; 0.1–100 µL with 100-µL syringe
Injection Methods
Full-loop; partial-loop injections with low-dispersion injections, and microliter pickup
Injection Technique
Needle in needle, with programmable needle wash
Injection Precision
RSD <0.4% for 1 µL full-loop injections;
RSD <1% for 200 nL partial-loop injections
Linearity
Corr. Coeff. >0,9995, from 100 nL to 500 nL partial-loop injections
Carryover
<0.02% with needle wash
Injection Cycle Time
<30 s for 1-µL full-loop injection
Optional Sample Thermostatting
4–45 °C, at least 22 °C below ambient
Wetted Parts
PEEK, stainless steel, PCTFE, fused silica
Dimensions (h × w × d)
40 × 42 × 51 cm (16 × 16.5 × 20 in.)
Weight
23.8 kg (52 lb); 31.4 kg (69 lb) including cooling
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