Purification/powderization after retention and concentration of fractions of target compounds obtained by a preparative LC system "Crude2Pure" System with a completely new way
When purifying target compounds from extracts of natural products and synthetic substances via preparative LC, powderization of the target compounds of the fraction is an important process. Ordinarily, it takes time to purify and powderize the target compounds obtained by the preparative LC. It is also difficult to recover high-purity powder.
The Crude2Pure system, which is based on ground-breaking purification methods, provides a new approach that reduces the work of the purification/powderization process after preparation.
Points
- Removing Solvent-derived Components and Recovery of High-Purity Powder
- Fast Powderization of Liquid Fractions
- Setting Samples at Any Time – Open Access –
The Crude2Pure system consists of a trapping system equipped with a dedicated Shim-pack C2P-H column for trapping and concentrating the target compound, and a recovery system that automatically powderizes and recovers the trapping target compound.
Single and multi-system configurations are available to meet specific operational requirements.
Retention and Concentration of Compounds —Crude2Pure Trapping System—
Ordinarily, when removing solvents, it is necessary to evaluate detailed conditions for each compound. With the Crude2Pure, however, simply place the preparative LC fraction in the autosampler, and enter the compound information into the interactive software to automatically trap the compound. In addition, the newly developed Shim-pack C2P-H trapping column can be used with a wide range of organic solvents and pH conditions, and provides high loading and high recovery rates. Further, trapping conditions are determined from the information on retention times obtained during fractionation, so there is no need to evaluate conditions individually. This eliminates the need to perform difficult preliminary evaluations to determine trapping conditions.
Powderizing Compounds —Crude2Pure Recovery System—
This stage recovers the target compound trapped in the trapping column and then simultaneously powderizes it. In addition, it can perform desalination and salt substitution as is, prior to recovery.
The sequence from pretreatment to powderization takes approximately three to four hours, which saves a great deal of time in comparison to centrifugal concentration or freeze drying. The vial in which the powderized target compound is recovered can be used as a storage container as is, eliminating the need to transfer the samples. Furthermore, adding the changer option increases the throughput of drying and powderization via 6-line parallel processing (multi system).
