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Instrument Database:
Bruker Daltonics - micrOTOF-Q
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| Year of introduction |
2005 |
| Status |
historical ( out of sale ) |
| Company |
Bruker Daltonics
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| Categories |
Hyphenated Techniques: LC-MS
Mass spectrometer: ESI-MS
Mass spectrometer: LC-MS
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Unique power3 for discovery- Precise mass
- SigmaFit™
- MS/MS
The micrOTOF-Q uniquely offers three dimensions of identification information simultaneously on all results: precise mass, MS/MS and SigmaFit™ isotopic analysis for unprecedented certainty in discovery science.A compact footprint of only 64 x 94 cm (25" x 37"), yet the instrument is market-leading in performance among all the benchtop and floor-standing ESI-Qq-TOF systems.The new electrospray orthogonal time-of-flight mass spectrometer is perfectly equipped for the requirements of Metabolomics, Proteomics and Drug Development. Easy formula determination of small molecules, metabolic studies, analysis of complex mixtures, digests and in-depth evaluation of intact proteins are key applications.Ultimate Sensitivity from Next-generation Ion Funnel ESI sourceThe new Apollo II™ Electrospray ion source with its advanced optics dramatically improves ion transmission.High-performance Q-q-front endThe micrOTOF-Q features a superb quadrupole mass filter and a quadrupole collision cell for accumulation of parent and fragment ions prior to mass analysis. The entire range of fragment ions is available at increased sensitivity for the time-of-flight mass analysis.Unequaled Mass Accuracy over a wide dynamic rangeThe micrOTOF-Q reaches a mass accuracy of better than 3 ppm by brilliant time-of-flight systems with innovative detection technologies. The instrument shares the famous stability of micrOTOF over a wide dynamic range without tedious recalibration routines. The mass position is kept stable for hours during temperature changes and over a wide dynamic range with significant variations in sample concentration.TIP – True Isotopic Pattern with SigmaFit algorithmAnalysis of the isotope profile provides the so-called “True Isotopic Pattern” (TIP™) which is matched against the measured spectrum. SigmaFit presents the correlation between measured and theoretical isotopic pattern, leading to results with significantly increased confidence. This unique combination significantly reduces the number of possible formula candidates and leads to unequivocally determination of elemental compositions of compounds.MS/MS performanceBringing together the unprecedented features of Bruker Daltonics micrOTOF - the excellent mass accuracy on fragments and superb focus resolving power exceeding 15,000 FWHM - with flexible data dependant MS/MS strategies, the micrOTOF-Q allows de novo sequencing of peptides even for post-translational modifications. Confident results in proteomics research meets accurate mass and structural analysis of small molecules.A software environment for maximum productivityCompass™, Bruker Daltonics unified software environment for all life science instruments, handles all system controls, allows full data management and provides full automation of the LC/MS workflow.Technical DataCutting-edge micrOTOF-Q technology- World-leading combination of mass accuracy, resolution and sensitivity without compromise
- SigmaFit™, the unique combination of accurate mass with True Isotopic Pattern (TIP™)
- Wide dynamic range for ultra-stable accurate mass
- High-performance Q-q-front end
- Footprint 640x949x1320 mm, weight 160 kg
Source options- Apollo II ion funnel ESI Electrospray source (1 µl – 1 ml/min)
- APCI atmospheric pressure chemical ionization source (100 µl – 1,5 ml/min)
- APPI atmospheric pressure photo ionization source
- online / offline NanoElectrospray source
- CE/MS coupling with grounded ESI needle
Analytical performance- Mass range 20 – 40,000 m/z
- Mass accuracy in MS and MS/MS 3 ppm RMS Error (internal), 5 ppm RMS Error (external)
- Mass resolution in MS and MS/MS 15,000 (FWHM)
- Temperature compensated
- Acquisition rate (2GHz sampling rate) 20 Hz (profile and peak detected spectra to disk)
Compass software suite- Integrated LC-MS/MS control and data processing
- Generate formula module using SigmaFit
- BioTools™/RapiDeNovo™ software for protein data interpretation
- ProteinScape™ database system for proteome project management
- MetaboliteTools™ software for metabolite identification
- ProteinScape™ database system for proteome project management
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