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Instrument Database:
Prince Technologies B.V. - PrinCE 550 and 560 Capillary Electrophoresis System
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| Year of introduction |
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| Status |
historical ( out of sale ) |
| Company |
Prince Technologies B.V.
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| Categories |
Separation: Electrophoresis, Capillary
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PrinCE 550 and 560: One of the most flexible
The PrinCE 550 and 560 are one of the most flexible capillary electrophoresis systems. Both these systems are added with a second lift. This second lift is used to allow the change of outlet buffers at will.
Because of the dual lift approach, the PrinCE 550 and 560 is the most versatile of all CE instruments without external pressure possibilities. It is ideally suited for method development and routine analysis by one or more methods after another. The PrinCE 560 offers the possibility of sample and buffer cooling. Further the injection can be performed from short end. This reduces the effective capillary length. If the detector is used with a remote detector cell that fits into the oven compartment of the PrinCE, the capillary length can be reduced considerably.
Both PrinCE systems are fully automated and can handle up to 32 (or optional 40) samples without interference of the operator, while still maintaining 10 (or optional 5) buffer vials. Any combination of six segments (sample/buffer type) can be used. The operator can exchange the segments easily without needing any tools.
Under precisely controlled circumstances it is possible to use the PrinCE 550 and 560 for fraction collection.
The detector will typically be placed on top of the PrinCE, which will reduce bench space.
The extra features of PrinCE 560, compared to the PrinCE 550:
- Samples and Buffers cooling
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| Specifications |
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| Injection modes | Hydrodynamic injection uses patended Dynamic Compression Injection (DCI) to generate and apply a range of positive and negative pressures with controlled ramping (-180 to 250 mbar, 1 mbar resolution). Electrokinetic injection features controlled ramping in voltage or current mode (max. 30 kV) | | Flushing pressure | 0 to +3000 mbar by DCI device | | Voltage range | -30 kV to +30 kV | | Current range | -200 µA to + 200 µA | | Ramping | Programmable voltage or current ramping | | Polarity reversal | Programmable pre-run and during the run | | Autosampler | 30/48 position carousel sample and buffer vials. Variable vial sizes of samples and buffers available, including Eppendorf micro centrifuge tubes. | | Buffer volume | Maximal 4 ml | | Sample volume | Minimal 10 µl, maximal 4 ml | | Temperature control | Samples & buffers cooling 4°C to 40°C (PrinCE 560 only). Capillary: 5°C below ambient to 60°C | | Capillary | Flexible in length and diameter depending on application or configuration | | Sophisticated features | Current or voltage controlled method step durations (for on-line pre-concentration). Positive or negative pressure during electrophoresis. Outlet-end injections of sample or reagents. | | Power requirement | 115/230 V, 50/60 Hz, 300 VA | | Dimensions | 50 x 50 x 54 cm (w x h x d) | | Weight | 35 kg | | Accessories | Detector table on top, WinPrinCE control software and a start-up kit of sample, buffer vials and so on | | Detector | Allows the use of any CE- detector including UV/VIS, Fluorescence, Diode Array, LIF, Conductivity, Electrochemical and MS. | | Software | Allows the use of any data station. Optional: Our DAx Data Acquisition and Analysis software. This software is specially made for our PrinCE-systems |
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