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Instrument Database:
Agilent Technologies Inc. - G1321A (Series 1200) Fluorescence Detector
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| Year of introduction |
2006 |
| Status |
available |
| Company |
Agilent Technologies Inc.
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| Categories |
Chromatography: LC-Detector: Fluorescence
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Sensitive reliable fluorescence detection
As a standalone detector or in an automated Agilent 1200 Series system the Agilent 1200 Series fluorescence detector brings high-sensitivity fluorescence detection to your lab. This easy-to-use detector provides quantitative data and fluorescence spectra from a single run. Simultaneous multi-wavelength detection improves sensitivity and selectivity. Use the spectral information for rapid method optimization and verification of separation quality.
Features
- Superior sensitivity for lower detection limits at any combination of excitation and emission wavelengths . A long-life flash lamp, simplified optics and a reference system improve baseline stability.
- Improved selectivity and sensitivity by simultaneous multiple wavelength detection. Monochromators work in signal or spectral mode and are synchronized with the flash light to allow for detection at up to 4 excitation or emission wavelengths.
- Specification for dual wavelength operation now also available.
- Method optimization or peak confirmation by exploring the spectral landscape. View online spectra without interrupting your chromatography.
- Fast offline characterization of new compounds - Fill the FLD cuvette with your pure compound and take a fluorescence scan to get a complete visualization of all excitation and emission spectra with a single task.
- Verify wavelength accuracy for GLP compliance - Automatically check the built-in wavelength calibration (Raman band H20) with external standards that your wavelength is indeed what the setpoint says it is.
- Built-in diagnostics software and long-life lamp.
- Easy front access to flow cell for fast inspection or exchange.
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| Specifications |
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| Specifications Agilent 1200 Series Fluorescence Detector | | Performance | Single wavelength operation:
10 fg anthracene LOD, Ex 250 nm, Em 400 nm
H20 >500 Raman, Ex 350 nm, Em 397 nm,
dark value 450 nm, standard flow cell
Dual wavelenght operation:
H20 > 300 Raman, Ex 350 nm, Em 397 nm and Ex 350, em 450 nm, standard flow cell | | Light source | 20 W Xenon flash lamp, normal mode: 20 W
economy mode: 5 W | | Pulse frequency | 365 Hz for signal mode, 73 Hz for economy mode | | Excitation monochromator | Concave holographic grating, F/1.6, blaze 300 nm, range 200-700 nm and zero-order, bandwidth 20 nm | | Emission monochromator | Concave holographic grating, F/1.6, blaze 400 nm, range 280-900 nm and zero-order, bandwidth 20 nm | | Photomultiplier | 200-600 nm, exchangeable | | Reference system | Inline excitation measurement | | Time programing | Up to four signals, response time, PMT gain, baseline behavior (append, free, zero), spectral parameters | | Spectral acquisition | Excitation or emission spectra, scan speed 28 ms per datapoint, e.g. 0.6 s per spectrum 200-400 nm, 10 nm step | | Step size | 1-20 nm | | Spectra storage | All | | Wavelength repeatability | ±0.2 nm | | Wavelength accuracy | ±3 nm | | Flow cells | Standard: 8 µL volume, 20 bar (2 MPa) pressure maximum, quartz.
Optional: fluorescence cuvette for offline spectroscopic measurements with 1 mL syringe, 8 µL volume, quartz | | Control and data evaluation | Agilent ChemStation for HPLC or Agilent 1100 Series control module without spectral data analysis | | Environment | 0 - 40 °C constant temperature at <95% humidity (non-condensing) | GLP | Early maintenance feedback (EMF) for continuous tracking of instrument usage, display of feedback messages in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy using Raman band (H20) lines. |
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