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Instrument Database:
Thermo Scientific - Q Exactive™ Hybrid Quadrupole-Orbitrap™ Mass Spectrometer
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| Year of introduction |
2012 |
| Status |
available |
| Company |
Thermo Scientific
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| Categories |
Hyphenated Techniques: LC-MS
Mass spectrometer: ESI-MS
Mass spectrometer: LC-MS
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Identify, quantify and confirm more compounds rapidly and with confidence using the Thermo Scientific™ Q Exactive™ Hybrid Quadrupole-Orbitrap Mass Spectrometer. This benchtop LC-MS/MS system combines quadruple precursor ion selection with high-resolution, accurate-mass (HRAM) Orbitrap detection to deliver exceptional performance and versatility. The Q Exactive Mass Spectrometer is equally useful for untargeted or targeted screening and a broad range of qualitative and quantitative applications in drug discovery, proteomics, environmental and food safety, clinical research and forensic toxicology.
High-Confidence Results, Quickly
- HRAM and full-scan capabilities capture all sample data, all the time, enabling retrospective data analysis without need to re-run samples
- Resolving power up to 140,000 FWHM eliminates isobaric interferences, increasing confidence in results when analyzing samples in complex matrices
- Better than 1ppm mass accuracy in full-scan and all-ion fragmentation (AIF) modes ensures confident compound identification
- Fast scanning at 12hz and spectral multiplexing suited to UHPLC applications
- Rapid polarity switching maximizes information obtained
- AIF and multiple dissociation techniques—in-source CID and HCD—aid in compound identification
- Extended mass range to 6,000m/z enhances detection of singly charged small molecules and biomolecules
- More than four orders of magnitude intrascan dynamic range, along with femtogram-level sensitivity, allow detection of trace-level and high-abundance compounds in the same scan
Greater Confidence for Drug Discovery and Metabolism Studies
- Triple quadruple-equivalent ability to quantify parent compounds and metabolites along with identity confirmation provides greater throughput
- Fast HRAM Orbitrap technology eliminates interferences for high-confidence metabolite structural identification and large-scale profiling of metabolomics and lipidomics samples
- Fast scan-to-scan polarity switching in both MS and MS/MS modes maximizes metabolite detection and identification from a single injection
More Information for Superior Proteomics
- HCD yields superior fragmentation and higher-quality MS/MS spectra compared to CID, improving identifications
- High spectral quality and a wide dynamic range make it possible to identify and quantify proteins other instruments cannot find.
- Quan/qual capability make it easier to transition from proteomic discovery experiments to targeted protein quantification
Highly Specific Environmental and Food Safety Analyses
- Specificity exceeding triple quadruple Q-TOF or Q-Trap levels make this mass spectrometer the preferred quan/qual screening platform
- Data-dependent, full-scan MS/MS capabilities and HRAM data ensure the highest possible confidence in every identification
High-Confidence Quanfirmation for Clinical Research and Forensic Toxicology
- The combination of SRM and HRAM capabilities provides high-confidence quanfirmation of endogenous steroids, drugs of abuse and pain-management therapeutics with ease.
Application-Specific Software for Greater Throughput
- Thermo Scientific™ ExactFinder™, LCQuan™, Mass Frontier™, MetQuest™, MetWorks™, SIEVE™ and TraceFinder™ software are available to speed data analysis.
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| Specifications |
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| Max. Resolving Power | 140,000 @ m/z 200 | | Max. Scan Rate | Up to 12 Hz at resolution setting of 17,500 @ m/z 200 | | Mass Accuracy | Internal: <1ppm RMS
External: <5ppm RMS | | Sensitivity | Full MS: 500fg Buspirone on column S/N 100:1
SIM: 50fg Buspirone on column S/N 100:1 | | Dynamic Range | >5000:1 | | Quadrupole Mass Filter | Thermo Scientific HyperQuad mass filter provides superior and unique combination of resolution and sensitivity
Variable precursor isolation width selection from 0.4 Da to full mass range | | Polarity Switching | One full cycle in < 1 sec. (one full scan positive mode and one full scan negative mode a resolution setting of 17,500) | | Multiplexity | up to 10 precursors/scan |
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