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Instrument Database:
ESA Inc. - Model 540 MicroTiter HPLC Autosampler
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| Year of introduction |
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| Status |
historical ( out of sale ) |
| Company |
ESA Inc.
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| Categories |
Automation: Autosampler (LC)
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- Versatile sample delivery uses vials or multi-well plates.
- Designed to utilize the popular microtiter plate format
- Three Injection Modes - flushed loop, partial loop and microliter pick-up for excellent reproducibility and no sample loss when dealing with limited sample volumes.
- Biocompatible , non-metallic flow path; stainless steel also available.
- Automated derivatization and diluting capabilities provide flexible formats for sample preparation.
- Uses 96, 96 deep and 384 well microtiter plates or up to 48 -1.5 mL sealed vials.
- Optional Peltier sample temperature control for cooling of sample vial/plates.
This versatile, multi-function HPLC autosampler can access samples from microtiter plates and standard autosampler vials. This unit can accommodate 96 and 384 standard and deep well micro plates, or up to 48 standard sample vials in a supplied rack. Sample cooling is available on selected models. Biocompatible fluidics are standard with optional stainless steel plumbing. It incorporates three selectable injection modes: full loop, partial loopfill and microliter pick-up. Sample processing capabilities are provided for sample mixing, dilution and on-line sample derivatization.
An optional plate feeder can fully automate sampling from as many as seven standard plates and 3 deep well plates.
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| Specifications |
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Sample Capacity:
1 well plate (128 x 86 mm) plus 4-10 mL vials for reagents, diluents and transport liquid or 48-1.5 mL vials (in 48 position vial rack)
Mutli-well Capacity:
96 well microtiter plate (std. or deep well), 384 well microtiter plate
Injection Volume:
0.1 - 1000 µL in 0.1 µL increments
Injections/Vial:
Maximum of 9
Analysis Time:
Maximum 9 hr 59 min. 59 sec.
Syringe:
250 µL (std.), Optional- 100 µl, , 500 µL, or 1000 µL
Needle Wash:
Programmable, 300-9999 µL, 250 mL wash solvent solvent bottle
Reproducibility:
Flushed Loop - <0.3% RSD; Partial Loop - <0.5% RSD; µL Pick-up - <1.0% RSD
Memory Effect:
<0.01% with programmable needle wash
Power Requirements:
110V or 230V ±20% selectable on rear panel, 50/60Hz
Communication:
Outputs - inject marker, well marker, labeled well marker, stop I/O (all relay & TTL), BCD (TTL), 4 auxiliary (relay), 4 programmable outputs (relay)
Inputs - next injection marker, next well marker, stop I/O, 4 programmable inputs (all TTL)
Certifications:
CE
Dimensions:
11 in. (W) x 15.7 in. (D) x 17.3 in (H)
(280 mm x 400 mm x 440 mm)
Weight:
22 kg (48 lbs.)
Factory Installed Options
Sample Temperature Control: Built-in Peltier control 4-40° C (factory installed)
Automatic Plate Feeder: Hold up to 6 std. microtiter plates or 2 deep well plates in addition to 1 plate on autosampler's deck.
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