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Instrument Database:
ESA Inc. - Model 540 HPLC Autosampler
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| Year of introduction |
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| Status |
historical ( out of sale ) |
| Company |
ESA Inc.
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| Categories |
Automation: Autosampler (LC)
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Extremely Versatile Automatic Sample Injection for HPLC
- Unique Injection System - a double needle system utilizing a stainless steel needle to penetrate the septum and then second needle for sample aspiration.
- Three Injection Modes - flushed loop, partial loop and microliter pick-up for excellent reproducibility and no sample loss when dealing with limited sample availability.
- Automated Derivatization and Diluting Capabilities - flexible formats for sample preparation.
- Peltier Sample Temperature Control - integral heating and cooling of the sample racks.
- Flexible Vial Format - supports a wide range of sample vial shapes and sizes.
- Automatic vial sensor system detects vial presence and size.
- Ideal for Microdialysis studies.
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| Specifications |
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Sample Capacity:
96 1.5 mL vials, 72 4.0 mL vials, 160 0.5 mL vials, 32 10 mL vials; Sample rack consists of 8 segments which can include any combination of formats
Injection Volume:
1 - 1000 µL
Injections/Vial:
Maximum of 9
Analysis Time:
Maximum 9 hr 59 min. 59 sec.
Syringe:
100 µl, 250 µL, 500 µL, or 1000 µL
Needle Wash:
Programmable, 250 mL wash solvent bottle built in
Reproducibility:
Flushed Loop - <0.3% RSD; Partial Loop - <0.5% RSD; µL Pick-up - <1.0% RSD
Memory Effect:
<0.01% with programmable needle wash
Power Requirements:
110V or 220V selectable on rear panel
Communication:
Outputs - inject marker, vial marker, labeled vial marker, stop I/O (all relay & TTL), BCD (TTL), 4 auxiliary (relay), 4 programmable outputs (relay), 4 bit time base (TTL)
Inputs - next injection marker, next vial marker, stop I/O, 4 programmable inputs (all TTL)
Dimensions: 11 in. (W) x 21.3 in. (D) x 17.3 in (H)
(280 mm x 540 mm x 440 mm)
Weight:
22 kg (48 lbs.)
Options
Sample Temperature Control: Built-in Peltier control 4-40° C (factory installed)
Integrated Stream Switching: Two optional high pressure 6 port valves (factory installed)
Micro Version: Special injection valve and needle; injection range: 0.1 µL to 2.5 µL; comes with sample temperature control
Micro-vial Tray: 160 position for 0.5 mL vials
LSV Tray: 72 positions for 4.0 mL vials
Super LSV Tray: 32 positions for 10 mL vials
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