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Instrument Database:
Agilent Technologies Inc. - G1382A (1200 Series) Capillary Pump
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| Year of introduction |
2006 |
| Status |
available |
| Company |
Agilent Technologies Inc.
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| Categories |
Chromatography: LC-Pump
Chromatography: LC-Pump: Capillary
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For capillary LC and LC/MS applications
The Agilent 1200 Series capillary LC pump offers better control and flexibility of low flow rates. The pump is ideally suited for capillary LC and LC/MS applications, like bio-pharmaceutical research, where sample volumes are limited and higher sensitivity is essential. Application focus: Capillary LC is frequently used for the analysis of drugs and its metabolites to gain sensitivity, especially when sample volume is limited. Important for low flow rate ranges is a robust and reliable instrument. It provides a stable flow rate throughout the analysis – capillary LC as easy as standard LC.
Features
- Reliable and reproducible results - using Electronic Flow Control (EFC) that provides real-time adjustment of the flow rate throughout the analysis; offers accurate flow rates even with solvent mixtures.
- Highest flexibility - In capillary mode optimized flow rates from 1 µL/min up to 100 µL/min (in normal mode up to 2.5 mL/min with additional hardware changes).
- Maximum performance - Retention time (RT) stability independent from column back pressure.
- Even more flexibility on solvent selection by using the built-in solvent selection valve that combines two out of four solvents for binary gradient formation, or select a different solvent for flushing the column.
- Low delay volume through the use of micro volume components.
- Low detector baseline noise and long-term stability using the included micro vacuum degasser.
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| Specifications |
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| Agilent 1200 Series Capillary Pump | | Hydraulic system | Two dual pistons in series, with proprietary servo-controlled variable stroke drive, floating pistons and active inlet valve, solvent selection valve and electronic flow control (EFC) for flow rates up to 100 µL/min | | Settable column flow range | 0.01 – 20 µL/min, 0.01 – 100 µL/min (with extended flow range kit), 0.001 – 2.5 ml/min (electronic flow control bypassed) | | Recommended column flow range | 1–20 µL/min, 10–100 µL/min (with extended flow range kit) | | Column flow precision | < 0.7 % RSD or 0.03 SD, whatever is greater (typically < 0.4 % RSD or 0.02 SD), at 10 µL/min and 50 µL/min column flow (based on RT, default settings) | | Composition precision | < 0.2 % SD at 10 µL/min, 50 µL/min (with extended flow range kit) and 1 ml/min (default settings) | | Pressure range | up to 400 bar system pressure | | Delay volume | Typically 3 µL from EFC to pump outlet for flow rates up to 20 µL/min; 12 µL from EFC to pump outlet for flow rates up to 100 µL/min; 180–480 µL (system pressure dependant) without mixer for flow rates up to 2.5 ml/min; mixer delay volume 420 µL | | pH range | 1.0 to 8.5 recommended |
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