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Instrument Database:
Bio-Rad Laboratories - BioLogic LP System
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| Year of introduction |
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| Status |
available |
| Company |
Bio-Rad Laboratories
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| Categories |
Chromatography: LC/HPLC
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The BioLogic LP low-pressure chromatography system is ideal for biomolecule purification. It brings high performance, versatility, ease of use, and affordability to low-pressure applications. The compact design maximizes available workspace in the coldroom and on the laboratory bench. The BioLogic LP system includes high-performance features such as:
- Easy-to-use LP Data View software designed for the BioLogic LP system; the software captures data, multitasks, and prints data from any computer that runs on Microsoft Windows 95, 98, MC, NT version 4.0, or 2000 operating systems
- Methods storage — The system stores up to 50 methods; each method can include up to 50 pump steps and 50 fraction collection steps
- Buffer selection — Select up to 5 buffers and completely automate sample separation with the addition of an SV-5 buffer-select valve; the valve can also be used to automatically load large sample volumes
- A high-flow pump — The system houses a peristaltic pump with a flow rate range of 0.05–40 ml/min and maximum backpressure of 30 psi. The system is compatible with Econo-Column low-pressure chromatography columns, Macro-Prep chromatography media, Econo-Pac cartridges, Pharmacia HiTrap cartridges, RESOURCE columns, SOURCE media, and all other low-pressure chromatography media
- Detection capabilities — The system includes both 280 and 254 nm filters for protein and nucleic acid detection, and a conductivity cell to monitor gradient progress
- Fraction collection — The system offers both simple and sophisticated fraction collection choices: collect into 13 x 100 mm tubes or microtubes using the Model 2110 fraction collector, or collect into virtually any size container, from microplates to carboys, using the advanced BioFrac fraction collector; in addition, the BioLogic LP system supports the use of many non-Bio-Rad collectors
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| Specifications |
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Pump
Flow range 0.1–40 ml/min
Maximum counterpressure 30 psi (2 bar)
Gradient formation Solenoid-activated proportioning valve
Mixer volume: 750 µl
Detection Performance
UV Single channel, 280/254 nm with auto zero
Detection range: 0.001–2 AUFS
Conductivity Detection range: 0.5–500 mS/cm FS
Temperature Range 4–40°C
Sample Loading Manual loop fill
Automatic loading through SV-5 buffer select valve
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