Eksigent - NanoLC-2D system

Eksigent’s second-generation NanoLC™ system delivers stable, reproducible separations for peptide/protein identification, phosphopeptide analysis, biomarker discovery, metabolomics, and more.


The NanoLC-2D is a two-dimensional nano HPLC that lets you set-up a 2D method in seconds. The system comes with an optional autosampler for rapid injection of large sample volumes supporting full loop, partial loop, and zero sample loss modes. The system easily integrates with Thermo Electron’s Xcalibur® software, Bruker Daltonics’ HyStar® system and ABI/Sciex Analyst® software.

Identify more proteins from complex mixtures with automated 2-D chromatography

The NanoLC-2D system provides all of the performance capabilities of the NanoLC-1Dplus with the added capability of fully automated 2-dimensional separations. The result is high sensitivity, fast column format changes, low-solvent waste, and highly reproducible separations.

The NanoLC-2D system is composed of two complete binary-gradient nanoflow chromatography systems that can be configured for automated two-dimensional separations or as parallel, independent binary gradient HPLC systems. With its revolutionary pumping system, you can operate in three modes: 1D, 2D, or parallel separations.

NanoLC system features-at-a-glance

• Optimized for ultra-low flow rates of first dimension 1-20 µL/min, second dimension 50-1000 nL/min.
• Novel pumping system eliminates the need for flow splitting, providing excellent run-to-run reproducibility.
• Use automated peak parking for extended MS/MS analysis to improve peptide sequence coverage.
• Take advantage of nanospray sensitivity.
• Active flow control of each mobile phase ensures precise gradient delivery.
• Quickly responds to changes in flow rate set point in less than two seconds.
• Reduce solvent consumption by 99%!

system components

• 2 independent binary gradient pump systems
• 10-port column switching valve, accommodating 2 trap columns and an analytical column
• Integrated temperature-controlled autosampler (96- or 384-well plate compatible) with a 6-port biocompatible sample injection valve

flow rate range

• First dimension: 1-20 µL/min
• Second dimension: 20-1000 nL/min

pump type
Microfluidic direct pumping system with independent flow control feedback for each mobile phase Retention time RSD < 0.5% at 500 nL/min.

gradient formation
High-pressure gradient mixing; no flow splitting

delay volume

• First dimension: 300 nL from mixer to column
• Low-flow dimension: 65 nL from mixer to column

delay time
< 25 sec at 500 nL/min

mobile phase compatibility
All mobile phases compatible with 316 stainless steel, PEEK, and silica

injections
Standard, metered and rapid injection modes

injection volume
200-50 µL; option for larger sample volumes available

columns

• First dimension: optimized for use with 300-500 µm i.d. traps or columns (5 cm)
• Second dimension: 50-150 µm i.d. columns, 5-15 cm in length

peak parking
Response time from 500-100 nL/min typically < 4 seconds at spray tip. Response time can vary with external hardware configuration.

autosampler
One 96- or 384-well plate capacity Peltier type temperature maintained sample compartment. 5-40° C; cooling capacity 12° C below ambient Dual needle design for seal piercing Integral 6-port biocompatible injection valve.

system control
Computer with graphical user interface for control of all system parameters. Software allows import of run tables and creates CDF, text, and Excel files for data export and analysis. Tracking of instrument runtime, column usage, total injections, solvent usage, and error codes. System drivers available for Thermo Electron’s Xcalibur™, Applied Biosystems/MDS SCIEX Analyst®, and Bruker Daltonics’ HyStar™ mass spectrometer software.

power requirements
100-240 VAC, 4/63 Hz, 3A

dimensions

• Width: 18 inches (46 cm)
• Depth: 22 inches (56 cm)
• Height: 8 inches (20 cm)
• Computer: additional lab spaced needed for computer, keyboard, mouse, and monitor