The JASCO model HPLC FP-2020 fluorescence detector is recognized as the industry's most sensitive detector with a signal-to-noise ratio of better than 350:1 for the Raman water peak.
The FP-2020 fluorescence detector covers a wide wavelength range both for excitation and emission from 220 to 700 nm (~ 900 nm with an optional PMT) with proven stability.
Versatile time-programming capabilities are provided for wavelength, response, gain, spectral scan, etc., permitting highly selective detection of various compounds. A rapid scan feature allows on-the-flow spectral acquisition for both emission and excitation spectra without interrupting the chromatographic elution. A specially designed chemiluminescence attachment and capillary electrophoresis cell are optionally available. The FP-2020 can easily be interfaced with the JASCO HPLC system using the new 32 bit HSS-2000 HPLC System software for Windows2000. The HSS-2000 package offers full automation of the JASCO HPLC system including the FP-2020.
Improvement in the optical design and coating allows greater efficiency in the collection of fluorescence energy resulting in detection of anthracene (S/N = 1) at levels as low as 40 femtograms. With a S/N of greater than 350:1, the FP-2020 is the most sensitive HPLC fluorescence detector on the market ensuring accurate detection and quantification for applications with very low compound concentrations.
Holographic concave diffraction gratings and non-spherical mirrors are cleverly incorporated in a compact package resulting in extremely efficient and reliable monochromators. Replacement of the lamp can be easily performed in minutes by the user without even opening the instrument cover.
A built-in thermistor monitors optical bench temperature and automatically compensates for temperature variation via feedback through the CPU. Digital filtering further enhances signal intensity producing highly-reliable quantitative data especially for applications requiring the ultimate in detection limits.
Rapid Spectral Scanning
Rapid spectral scan functions allow on-flow spectral scanning of both emission and excitation spectra without interrupting the chromatographic elution. The FP-2020 can store up to 10 EX and EM spectra within the on-board microprocessor. Spectral subtraction allows a true sample spectra by eliminating interference from the mobile phase solvent.
A variety of flow cells
In addition to the standard 16 µl flow cell, various type of flow cells, Micro flow cell (5 µl), Capillary flow cell for capillary electrophoresis, Chemiluminescence flow cell, Inert flow cell are available to meet various applications. A rectangular cell holder is also available to place a rectangular cell (10 mm x 10 mm) for use of the FP-2020 as spectrofluorometer for methods development and finding optimal EX and EM wavelengths.