NEMI Method summary: 130.01: Organic tin compounds in marine animal tissues by GC-FPD
Measurements of organotin compounds in biological tissues require the isolation of these contaminants from the matrices. An aliquot of the homogenized tissue sample is extracted three times by maceration with 0.05% tropolone in dichloromethane in the presence of sodium sulfate. The extract is concentrated by Kuderna-Danish technique and the solvent is exchanged to 10 mL of hexane. Organotin compounds are hexylated with Grignard reagent and the derivatized extract is purified using silica gel/alumina chromatography before instrumental analysis. Quality control samples are processed with each bath of samples in a manner identical to that of the actual samples.