Ecomar Industria de Pesca S/A (Vigia, Pará, Brazil) collected the candidate reference material in Pará, Brazil. The reference material was produced from Dourada fish
(Brachyplatystoma Flavicans). Circa 18 kg of Dourada fish, comprising nine fishes, was frozen (-20 C) and transported to the laboratory. The individuals were cut and minced using a domestic mincer (head and tail despised), then freeze-dried (MLW-LG05, Leipzig, Germany), ground by hand in a mortar and pestle, and sieved using a domestic polypropylene sieve with 250 µm.
keywords: biological material , animal tissue , foodstuff , fish/shellfish , trace elements , speciation analysis , mercury speciation