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The establishment of EVISA is funded by the EU through the Fifth Framework Programme (G7RT- CT- 2002- 05112).

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isotope-coded affinity tag (ICAT)

In order to establish the relevance of a protein to an organism’s phenotype, it is important to be able to determine quantitative changes in its expression. This can be achieved by labelling with a compound (tag) which contains stable isotopes  such as 2H. One batch of the organism is tagged  with the heavy form of the label, containing the  stable isotopes (2H), and another batch is tagged  with the light form, containing 1H. The reactions  are specific for cysteine residues within the  proteins. The labelled samples are mixed and  digested with trypsin and the isolated peptides are  analysed by LC/MS/MS, enabling the relative  abundances of each peptide pair, containing the  light and heavy label, to be determined. ICAT is a commercial term. 


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