About 350 kg of mixed fish species was collected in the eastern Irish Sea by the CEFAS, Lowestoft, U.K. The skin was removed, the fish filleted, freeze-dried and subsequently sent to IAEA-MEL for processing. The sample, which was reduced by freeze-drying to about 69 kg, was ground to powder, sieved through a 0.5 mm mesh and homogenized by mixing in a nitrogen atmosphere. The sample was freeze-dried once more, ground and sieved at 250 μm, the fraction above 250μm was grinded again by micronisation. After reprocessing the sample was 95% <160μm. Obtained powder was further homogenized and bottled in sealed plastic containers. keywords: biological material , animal tissue , foodstuff , fish/shellfish , trace elements , speciation analysis , mercury speciation
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