SRM 1947 - Lake Michigan Fish TissueStandard Reference Material (SRM) 1947 is a frozen fish tissue homogenate, which was prepared from fish collected from Lake Michigan, and is intended primarily for use in evaluating analytical methods for the determination of selected trace elements, methylmercury, total mercury, polychlorinated biphenyl (PCB) congeners, chlorinated pesticides, and polybrominated diphenyl ether (PBDE) congeners, proximates, caloric content and fatty acids in fish tissue and similar matrices. All of the constituents for which certified, reference, and information values are provided are naturally present in the fish tissue homogenate. SRM 1947 was prepared from fillets from adult lake trout (Salvelinus namaycush) collected in October 1997 at Fisherman’s Island and Big Reef near Charleviox, MI in northern Lake Michigan. The fillets were removed from the fish using stainless steel knives and placed in Teflon bags. The tissue was placed on wet ice and transported to NIST where it was stored in liquid nitrogen vapor freezers (−120 °C) until processed and bottled. A total of 79 kg of fillets was obtained. The frozen fillets were pulverized in batches of approximately 350 g using the cryogenic procedure described previously [3]. The pulverized fish tissue was then homogenized in an aluminum mixing drum in two batches of approximately 40 kg each [4]. The mixing drum was designed to fit inside a liquid nitrogen vapor freezer and to rotate in the freezer thereby mixing the frozen tissue powder. After mixing for 2 h, subsamples of approximately 8 g of fish tissue homogenate were aliquoted into pre-cooled glass bottles. keywords: biological material , animal tissue , foodstuff , Fish/Shellfish, trace elements , heavy metals, mercury speciation , speciation analysis |
