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Instrument Database:
Thermo Scientific - Exactive™ Plus Orbitrap Mass Spectrometer
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| Year of introduction |
2012 |
| Status |
available |
| Company |
Thermo Scientific
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| Categories |
Hyphenated Techniques: LC-MS
Mass spectrometer: ESI-MS
Mass spectrometer: LC-MS
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The Thermo Scientific Exactive Plus Orbitrap MS is a benchtop LC-MS system designed for high-performance, high-throughput screening, compound identification, and quantitative analysis. Thanks to its Orbitrap™ mass analyzer, the Exactive Plus™ system delivers high-resolution, accurate-mass (HR/AM) full-scan MS for fast, precise and reproducible results and tremendous analytical confidence. The continuous acquisition of HR/AM full-scan data allows retrospective data analysis for qualitative and quantitative analyses. Moreover, it vastly simplifies MS method development. The Exactive Plus mass spectrometer features fast scanning that makes it fully compatible with narrow UHPLC peaks. It also includes fast polarity switching, which can further streamline method development and increase sample throughput. All-ions fragmentation (AIF) with multiple dissociation techniques—in source collision-induced dissociation (CID) and higher energy collisional dissociation (HCD)—adds an additional dimension of information. High-Confidence Results, Quickly
- HR/AM and full-scan capabilities capture all sample data, all the time, enabling retrospective data analysis without need to re-run samples
- Resolving power up to 140,000 FWHM eliminates isobaric interferences, increasing confidence in results when analyzing samples in complex matrices
- Better than 1ppm mass accuracy in full- and all-ion fragmentation (AIF) scan modes ensures confident compound identification
- Fast scanning at 12hz and spectral multiplexing suited to UHPLC applications
- Rapid polarity switching maximizes information obtained
- AIF and multiple dissociation techniques—in-source CID and HCD—aid in compound identification
- Extended mass range to 6,000m/z enhances detection of singly charged small molecules and biomolecules
- More than four orders of magnitude intrascan dynamic range, along with femtogram-level sensitivity, allow detection of trace-level and high-abundance compounds in the same scan
Easy-to-Use
- HR/AM and full-scan capabilities simplify MS method development
- Intuitive operating software reduces learning curve
- Compatibility with Thermo Scientific™, ExactFinder™, LCQUAN™, Mass Frontier™, MetWorks™, MetQuest™, SIEVE™, and TraceFinder™ software speeds data analysis
Upgradeable to Q Exactive
- The Exactive Plus Orbitrap Mass Spectrometer can be upgraded on-site to the Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer with the addition of a high-performance quadruple for precursor ion selection.
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| Specifications |
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Mass Range
| 50 to 6000m/z
| Dynamic Range
| >5000 within a single positive and negative scan mode with guaranteed specified mass accuracy
| MS Scan Power
| Full scan, 'All Ion Fragmentation' by HCD, data dependent AIF
| Mass Accuracy
| <1ppm using internal calibration, <3ppm with external calibration
| Polarity Switching
| One full cycle in <1 sec. (one full scan positive )
| Sensitivity
| A flow injection of 500fg of Buspirone giving a S/N of better than 10:1 for the selected mass chromatogram of the [M+H]+ ion
| | Resolution | 140,000 at m/z 200 at a scan rate of 1.5Hz, minimum resolution 17,500 at m/z 200 at a scan rate of 12Hz |
Mass Range 50 to 6000m/z Type Exactive Plus LCMS Mass Spectrometer Dynamic Range >5000 within a single positive and negative scan mode with guaranteed specified mass accuracy MS Scan Power Full scan, 'All Ion Fragmentation' by HCD, data dependent AIF Mass Accuracy <1ppm using internal calibration, <3ppm with external calibration Polarity Switching One full cycle in <1 sec. (one full scan positive Sensitivity A flow injection of 500fg of Buspirone giving a S/N of better than 10:1 for the selected mass chromatogram of the [M+H]+ ion Resolution 140,000 at m/z 200 at a scan rate of 1.5Hz, minimum resolution 17,500 at m/z 200 at a scan rate of 12Hz - See more at: http://www.thermoscientific.com/en/product/exactive-plus-orbitrap-mass-spectrometer.html#sthash.GDZXI92G.dpuf
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