The BioLogic DuoFlow chromatography systems offer flexibility with multiple system configurations, many optional upgrades, and a common control software that is intuitive and easy to follow.
Upgradable Systems Add Capability As Your Needs Change. BioLogic DuoFlow modular components allow you to design your own biocompatible chromatography system to specifically meet both laboratory space and application requirements. As experimental requirements change, systems may be easily reconfigured and seamlessly upgraded with increased functionality such as higher flow rates, sophisticated detection capabilities, pH monitoring, trace comparison, and buffer blending.
BioLogic DuoFlow System Selection Guide The F10 workstation includes an F10 pump for a flow rate of 10 ml/min at 3,500 psi. The F40 workstation includes an F40 pump for a flow rate of 40 ml/min at 1,000 psi. Flow rates for each pump can be doubled with the addition of the BioLogic Maximizer valve system.
BioLogic DuoFlow IQ/OQ Bio-Rad offers qualification protocols and services for the BioLogic DuoFlow chromatography systems and their peripheral components, including the BioLogic Maximizer valve system for buffer blending, the BioLogic QuadTec UV/Vis detector, and the BioFrac fraction collector. Bio-Rad's IQ/OQ (installation qualification and operation qualification) protocols are designed to help custermers comply with US FDA regulatory requirements. Precedures are performed by factory trained and certified technicians using instruments and reagents traceable to NIST standards.
IQ service verifies that the BioLogic DuoFlow chromatography system is properly installed in your laboratory. OQ service tests the performance of the installed chromatography system and provides a record that confirms critical functions and safety features.
For more information on IQ/OQ for the BioLogic DuoFlow chromatography system, contact your local Bio-Rad sales representative.
Typical computer and monitor configuration. Actual specifications may vary.
* Pentium III processor, 40 GB hard drive, 512 MB RAM * Microsoft PS/2 IntelliMouse * SoundNIC and video 4 MB graphics performance accelerator * BioLogic DuoFlow software, version 5.1 * CD-RW 8X CD-ROM drive * Networkable for printer and file access * Windows XP operating system * 17" LCD monitor
0.01–10 ml/min in 0.01 ml increments, to 3,500 psi (233 bar, 23 MPa)
0.5–40 ml/min in 0.01 ml increments, to 1,000 psi (66 bar, 6.6 MPa)
Flow rate with Maximizer
1.0–20 ml/min in 0.02 ml increments, to 3,500 psi (233 bar, 23 MPa)
1.0–80 ml/min in 0.02 ml increments, to 1,000 psi (66 bar, 6.6 MPa)
0.01–10 ml/min = 2%
0.5–40 ml/min = 2%
* Dual-piston, positive displacement solvent delivery pumps with interchangeable pumpheads made of biocompatible PEEK * Compositional accuracy: ±1% * High-pressure dynamic mixing: MX-1 mixer volume without extenders is 263 µl, mixer volume is expandable to 750 µl and 2 ml with added extender barrels; Maximizer mixer volume without extenders is 750 µl, volume is expandable to 5 ml and 12 ml with added extender barrels * Built-in pressure transducer, volume 46 µl * Sound level <70 dB
UV, Conductivity, and pH Detection * Mercury lamp for fixed wavelengths 280/254 nm, auto zero, 0.0001–2 AUF detection range * 5 mm path length analytical flow cell (standard) * 2 mm path length preparative cuvette flow cell, optional, included with F40 pump kit * Linearity: 2% up to 2 AU at 254 nm * 214 nm detection kit with zinc lamp, optional upgrade * Wavelength filters include 365, 405, 436, 546 nm, options to mercury lamp UV detector * Conductivity monitor: range = 0–500 mS/cm, accuracy = ±2% full scale * pH monitor option includes flow cell, electrode, and signal import module (SIM) * SIM supports other detectors, e.g., refractive index, fluorescence
General * System fittings: 1/4–28 fingertight nuts and ferrules * Coldroom compatible * Tubing: PEEK, Tefzel, and Teflon * Solvent compatibility: 1 M NaOH, HCl and organic acids, 0.1 M H2SO4, 7 M urea, guanidine HCl, 100% MeOH, isopropyl alcohol, acetonitrile, 0.1% TFA, 1% detergent (including SDS, Triton X-100) * Wetted materials 100% biocompatible * Workstation dimensions: ~ 35 x 41 x 18 cm (W x D x H), 3-tray rack Increases height to 76 cm