WaveQuest: the CE4300 Dynamic Absorbance Detector (DAD) is an ultra high speed scanning powerful alternative to Photo Diode Array detectors. HPLC method development, purity determination, R & D and Variable Wavelength Monitoring are all possible with the highest quality. Dual wavelength monitoring and wavelength programming are also provided.
The design is based on a double beam spectro-photometer concept with ultra fast wavelength scanning over the range 190-700nm.
This dynamic scan provides UV spectra in only 200mS, previously only possible using the static optical system of a PDA detector.
Powerful signal processing software has been developed which enables very high quality “on-the-fly” scans to be triggered by eluting peaks without the peaks being degraded.
Spectral data is transferred by Cecil’s DataStream software to a PC workstation where the same software offers spectral manipulation and processing, library facilities and the export of data to applications such as Excel, word etc. All data may be stored on disk.
Eluting peaks may be integrated at the same time as purity and peak identification is carried out. Quantitative evaluation and reporting is carried out by Cecil’s CE4900 PowerStream software, which is combined with DataStream software.
One Detector Covers All UV Applications
A single beam Photodiode Array Detector (PDA), used for method development and R & D, will often be replaced by a dual beam UV variable wavelength detector for routine monitoring, when the developed method is adopted, because of the poor drift performance of single beam PDA detectors. Their performance can be as much as 20 times worse. The Dynamic Absorbance Detector has a double beam optical system with very high all round performance and the lowest drift available.
The CE4300 may therefore be used for method development, purity determination, etc. and also as a high performance UV detector for variable wavelength monitoring (VWM).
WaveQuest, which is less expensive than a PDA detector, therefore has a double cost advantage.
Universal UV/Visible Detector
WaveQuest the ultra fast scanning Dynamic Absorbance Detector (DAD) is a major advance in UV-visible detector design, offering the capabilities of a Photo Diode Array (PDA) detector with greatly improved performance when used for chromatogram monitoring.
Very high speed scanning of eluting peaks, “on the fly”, makes purity checks, the determination of optimum monitoring wavelengths for unfamiliar peaks (lambda max scans), and compound identification of peaks using library spectra all possible with considerable cost saving.
CE4300 Dynamic Absorbance Detector
WaveQuest the new CE4300 Dynamic Absorbance Detector (DAD) for ultra fast scanning, part of the Adept HPLC range from Cecil Instruments, offers all the spectral facilities of Photo Diode Array detectors, with better noise and drift performance, particularly important when used to monitor and quantify chromatograms.
The CE4300 has a double beam optical system in which the dispersed radiation is dynamically scanned across the detectors in the manner of a UV-Visible double beam spectrophotometer at ultra fast speeds – up to 1000nm/second.
All components of the detector – monochromator, optical system, lamp optics, detector optics and signal processing software have been carefully optimised to provide unprecedented performance in both wavelength scanning and single or multi-wavelength monitoring.
The lower noise level when monitoring a chromatogram makes accurate measurements at the highest sensitivities possible when measuring very low concentrations.
The lower drift, as much as 20 times lower, gives flat chromatogram baselines making integration of peaks more accurate, particularly at high sensitivities.
Taper Beam Optics
Taper beam optical imaging at the flowcell, with all transmitted energy reaching the detector, provides maximum energy collection. The result is minimal baseline wander due to changes in the solvent refractive index during gradient formation.
Performance Validation
Validation of detector performance is carried out conveniently, using the instruments standard software. A Holmium Oxide filter is automatically inserted in the optical path when the calibration routine is selected.
Bandwidth validation is automatic, using the Deuterium emission line at 656.1nm. Absorbance accuracy is checked using filters traceable to NPL standards.
