Asymmetric Flow Field-Flow Fractionation, also called AF4, is an innovative new separation method for the efficient separation and characterization of proteins, polymers and nanoparticles in a fast and gentle way.
The AF2000 MT from Postnova is the most advanced AF4 system on the market today and is the benchmark for the industry, indicating the maximum possible performance. The instrument shows a higher resolving power and a more advanced software system than every other device available. It is the most integrated system and the only one with the channel inside the housing.
The system has a large, nearly universal separation range. Nanoparticles can be separated from 1 nm to 100 µm and proteins, peptides, polymers from 103 to 1012 Da. The fast & gentle separation occurs based on the diffusion coefficient in an open flow channel without using ANY stationary phase as is utilized in the technology of SEC/GPC.
UNIQUE FEATURES OF AF2000 MT
Patented Channel Cartridge Design
The AF2000 MT, as all other Postnova AF4 systems as well, is equipped with a special patented flow channel using a channel cartridge and a channel holder for fast and easy exchange of membrane and system cleaning procedure. Depending on the application, the cartridge can be replaced by different other cartridges, which are available, analog to different HPLC columns in a chromatography system.
Complete Bio-Compatible System
The AF2000 MT is the only system which can be delivered as a completely inert bio-compatible and metal-free system with a ceramic frit instead of a stainless steel frit, PEEK pumps, PEEK/ PMMA channel and PEEK autosampler.
Totally Integrated One-Box-System
Completely integrated system with all important parts located inside the system housing, including flow channel. Professional one-box system for use in laboratories with high-level expectations concerning system handling and user-friendliness.
Patented Cross-Flow Control
Special patented cross-flow control, using a unique dual syringe pump module with non-pulsing cross-flow delivery for most precise separation conditions. Completely inert, metal-free cross flow path made of glass, PEEK and teflon/PVDF. Instant cross-flow adjustment without time-delay or hysteresis (flow over and under adjustments) known from other commonly used cross-flow control valves. Rugged cross-flow control insensitive to plugging without the need for fragile and corrosion sensitive valve needles. Cross flow range from 5 µL/min (with 100 µL syringe) up to 16 mL/min (with 2 mL syringe) for lowest and highest possible cross-flows of all FFF systems. Different syringes available, which can be exchanged to extend flow rate range.
Data Evaluation Software - NovaFFF Analysis
Postnova offers a special data evaluation software with unique features. Data files from different postova FFF system/techniques can be processed. Software allows to perform absolute size calculation based on FFF theory and size calculation based on calibration with particle size standards. Possibility to calculate size of small particles by using a concentration detector (UV / RI) when light scattering is poor/not showing a sufficient signal. NovaFFF Analysis is an additional validation tool providing particle sizes of aggregates which can be directly compared with particles sizes from light scattering.
Integrated Method Wizard Expert System
Postnova offers a special NovaFFF control software with integrated Expert System. Method Wizard for accelerated and automated method generation by simply entering in the approximate size of the analyte. Advanced Simulation Module which is also directly integrated in the control software and where the user can run a complete method in a simulation first. Run Parameter Optimisation Tool to optimize run conditions in terms of run time, maximum resolution, etc..
Patended FOCUS Technology
The AF2000 MT uses PostnovaLs patented FOCUS technology which assures constant and continuous flow through the detectors all the time. That's why the system has a higher recovery, higher resolution and greater flexibility than other systems. Now AF4 can be coupled to fl ow sensitive viscometers and RI detectors successfully.
APPLICATIONS OF AF2000 MT
- Biotechnology : Viruses, Aggregates, Cell Organelles, Bioparticles.
- Pharma : Proteins, Antibodies, Liposomes, Drug Delivery, Micelles.
- Environment : Humic and Fulvic Acid, Environmental Colloids, Clay Particles.
- Material Science : Latex Beads, Nanoparticle, High-Tech Nano Materials.
- Polymers : Biopolymers, Starches, Polyelectrolytes, Technical Polymers.