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Instrument Database:
Prince Technologies B.V. - PrinCE 700 Integrated Capillary Electro Chromatography system
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| Year of introduction |
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| Status |
historical ( out of sale ) |
| Company |
Prince Technologies B.V.
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| Categories |
Chromatography: LC/IC
Separation: Electrophoresis, Capillary
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The PrinCE 700 series is a powerful integrated Capillary Electro Chromatography system with a very sensitive Diode Array Detector (DAD). It is fully automated, flexible, modular and suitable for almost any application. Thanks to the original modularity of the PrinCE system, it is still easy to couple to any external CE detector, or to Mass Spectrometer (MS). The PrinCE 700 series also offers the possibility for external pressure up to 10 bars and is standard supplied with samples and buffers cooling. The integrated systems can handle up to 32 (or optional 40) samples without interference of the operator, while still maintaining 10 (or optional 5) buffer vials. Any combination of six segments (sample/buffer type) can be used. The operator can exchange the segments easily without needing any tools. Under precisely controlled circumstances it is possible to use the integrated PrinCE 700 series for fraction collection. Some features of the integrated PrinCE 700 series are:- Short effective capillary length: 7.5 cm, with total minimum capillary length of 33 cm
- Hardware selectable inlet and outlet for short or long effective capillary length
- Complete temperature control of capillary
- Capillary cassette
- Three types of DAD possible: 256, 512 or 1024 diodes
- Measurements in UV, VIS and NIR
- 3D analysis software, including: high resolution spectra, spectra library search, peak purity calculations, peak identification
- Low limit of detection
- Easy to couple to MS and other CE detector (like Conductivity, Fluorescense and others)
- Additional external fibre cell: offers the possibility to select between the standard internal fibre cell which goes with the PrinCE 700 series or select an optional remote fibre cell. This remote fibre cell is fully automated selectable and increases the flexibility of the PrinCE system and is ideal in combination with a MS because the sample can be measured very closely to the used MS. The cell with fibres can be connected to the “detector part” of the PrinCE 700 series using a set of panel fit SMA connectors. The additional external fibre cell is not standard available but optional as a factory set modification only.
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| Specifications |
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| Injection modes | Hydrodynamic injection uses patended Dynamic Compression Injection (DCI) to generate and apply a range of positive and negative pressures with controlled ramping (-180 to 250 mbar, 1 mbar resolution). Electrokinetic injection features controlled ramping in voltage or current mode (max. 30 kV) | | Pressure features | On inlet, outlet or both. | | Flushing pressure | 0 to +2000 mbar by DCI device, up to 10 bar by external pressure | | Voltage range | -30 kV to +30 kV | | Current range | -200 µA to + 200 µA | | Ramping | Programmable voltage or current ramping | | Polarity reversal | Programmable pre-run and during the run | Autosampler/
fraction collector | 30/48 position carousel sample and buffer vials randomly accessible from both capillary ends. Variable vial sizes of samples and buffers available, including Eppendorf micro centrifuge tubes. | | Buffer volume | Maximal 4 ml | | Sample volume | Minimal 10 µl, maximal 4 ml | | Temperature control | Samples & buffers cooling 4°C to 40°C
Complete temperature control of the capillary: 5°C below ambient till 60°C | | Capillary | Effective length: 7.5 cm, minimal total length 33 cm | | Sophisticated features | Current or voltage controlled method step durations (for on-line pre-concentration). Positive or negative pressure during electrophoresis. Outlet-end injections of sample or reagents. | | Power requirement | 115/230 V, 50/60 Hz, 300 VA | | Footprint | 61 x 45 x 48 cm (w x h x d) | | Weight | 45 kg | | Accessories | A start-up kit of sample and buffer vials | | Integrated DAD | Three different types of High sensitive Diode Array Detectors available:
256 diodes with a range from 190 nm up to 720 nm
512 diodes with a range from 190 nm up to 610 nm
1024 diodes with a range from 190 nm up to 1010 nm | | External Detector | Allows the use of any CE- detector including Fluorescence, Diode Array, LIF, Conductivity, Electrochemical and MS. | | External CE-cell | Allows the use of an external CE cell for measuring close to MS inlet | | Software | Our DAx-3D analysis software.
This software is specially made for our integrated PrinCE 700 series |
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