Instrument Database:
Shimadzu Europe - Prominence UFLCXR High-speed HPLC system
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| Year of introduction |
2008 |
| Status |
available |
| Company |
Shimadzu Europe
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| Categories |
Chromatography: LC/HPLC
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In Pursuit of Higher Speed and Uncompromised Separation
The goal of high speed HPLC is to enhance productivity of analyses in laboratory. We believe that analytical data obtained from high speed HPLC must be reliable at same level as those from conventional HPLC. For example, resolution must be kept at excellent level in case of 10,000 analytical runs by high speed LC , and time and area repeatability must be excellent for small volume sample injection, parts replacement is less required and the instrument must work stably.
We successfully achieved speed and separation performance levels that do not depend on high pressure, while simultaneously offering a high level of precision and expandability not available with previous fast LC systems.
The balance between an appropriately small particle-sized column media and the length of a column is an important factor to improve column performance. For true high-resolution analysis, a number of conditions must be met. These include finding a balance between increased column pressure and a smaller particle size; developing a system durable enough to handle the pressure; using a detector with high sensitivity and a wide dynamic range; and optimizing the flow path for minimal diffusion.
Prominence UFLCXR provides high-pressure endurance by utilizing the technological advancements and knowledge developed during the ongoing refinement of the Prominence series. Our aim has been to make high-speed analysis compatible with high resolution, not simply to perform high-speed analysis with a smaller particle-sized column. The Prominence UFLCXR achieved this goal.
High-speed LC analysis --- 10 times faster than conventional LC
The following is a comparison of chromatograms between Prominence UFLC with Shim-pack XR-ODS, a new reversed-phase column, and conventional LC using a conventional column (150mmL. x 4.6mm i.d., 5um). In conventional LC, benzo-fluoranthene isomers (peaks 7 and 8) with separation 1.2 were eluted in 35 minutes. Using Prominence UFLC with the Shim-pack XR-ODS, the same components were eluted in 3.5 minutes while maintaining the same excellent resolution. Retention time for benzo-perylene (peak 9) is reduced from 50.4 min. to 5.07 min., while its theoretical plate number changes slightly from 10,600 to 11,900.
Prominence UFLC reduces analysis time to 1/10 of conventional LC while keeping separation efficiency. (Analysis time reduction may vary by analytical conditions.)
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