nanoACQUITY UPLC: the Ideal Solution for Proteomics
The Waters nanoACQUITY Ultra Performance LC™ (UPLC™) System has been designed for capillary to nano-scale separations to attain the highest sensitivity, resolution and reproducibility possible for biomarker discovery, and for proteomics applications for protein identification and characterization.
The nanoACQUITY UPLC System allows scientists to achieve separations at nanoflow rates without flow-splitting, offering significant improvements in robustness, reproducibility and simplicity of operation over conventional nanoflow separations technologies.
Increased Peak Capacity Separations—at 10,000 PSI
Scientists are increasingly investigating large protein populations or proteomes to identify and quantify proteins that are either up-regulated or down-regulated.
These changes in protein expression may be due to, and are possibly indicative of, disease states. Identifying these subtle changes can provide valuable information for drug development. The best way to ensure that you get maximum information is to achieve better chromatographic resolution and increased peak capacity prior to mass spectrometric analysis.
The nanoACQUITY UPLC System takes advantage of 1.7 µm BEH particle chemistry and, with columns of up to 25 cm in length (running at back pressures of up to 10,000 psi) you benefit from a huge increase in chromatographic resolution.
Improve accuracy in qualitative and quantitative applications
The nanoACQUITY UPLC System is optimized for high-resolution 1D and 2DLC separations at precise nanoflow rates between 200 nl/min and 100 µL/min, on nanoACQUITY UPLC columns with internal diameters (i.d.) ranging from 75 µm to 320 µm. Retention time reproducibility for peptide mixtures is typically s.d. <0.20 min. — ideal for quantitative proteomics applications.
The example (Figure 1) shows a separation of protein digest standards on columns packed with 3.0 mm particles (top) compared with the same experiment using 1.7 mm BEH particles (bottom). Running the 1.7 mm column produces higher peak capacities, narrower and highly concentrated peaks, and more MS information.
Figure 1 - 5%B to 55%B in 30 minutes, 0.5 sec/spectra
5 protein MassPREP™ digest standard mixture, 20 fmol each of enolase, phosphorylase b, hemoglobin, ADH, BSA
Hardware and Software
The nanoACQUITY UPLC™ System directly benefits from the holistic design of our ACQUITY UPLC™ Systems. The instrument itself features ultra-low system and dwell volumes and takes full advantage of the proprietary 1.7 µm BEH separations technology for enhanced resolution of nanoscale UPLC separations.
The Waters nanoACQUITY UPLC System consists of:
- nanoACQUITY UPLC Binary Solvent Manager for direct-flow nanoscale gradient elution and sample trapping
- Proprietary nanoACQUITY UPLC Heating and Trapping Module, with nano-column management
- Auxiliary Solvent Manager, for higher flow rate column trapping and NanoLockSpray™ lock-mass addition
- nanoACQUITY UPLC Sample Manager
- Console that provides instrument control, status, and advanced diagnostic information
- Software and instrument control with MassLynx™ Software