|A complete line of ILE pipette tips (patent pending) is currently in development. ILE pipette tips utilize the same polymeric (methyl, fluoro, and phenyl-polysiloxanes) coatings as other ILE products. The polymeric coating is immobilized on a portion of the inner surface of ILE pipette tips. ILE pipette tips extract compounds from aqueous matrices by repeatedly aspirating a sample into and out of the pipette tip until extraction equilibrium is attained. The analytes are next "back-extracted" into a small amount of solvent by aspirating enough of that solvent into the ILE pipette tip to cover the coated portion of the tip, and then expelled with the solvent as a sample ready for GC or LC analysis. No emulsions, sorbent conditioning, sorbent clogging, nor solvent waste.
ILE pipette tips allow an analyst to perform extractions both manually and through the use of automated liquid handling systems. Automated liquid handling systems fitted with ILE pipette tips are able to extract without the requirement of any special hardware such as vacuum systems and collection plates, as in SPE. Additionally, protein precipitation is not required. Derivitizing reagents do not affect the coating, so back extraction and derivitization can be performed simultaneously. Tips will be available in 20 µL and 200 µL versions in standard 96 well packs.