This reference material is intended for use in the validation of new and existing methods, and monitoring the performance of methods, commonly used in clinical laboratories to determine the important trace elements copper, selenium and zinc in human serum samples. It can also be used in the training and evaluation of staff. The material is clinically relevant since it closely matches the lower level of the normal range for serum selenium in the population.
Human serum from a single donor was prepared from blood obtained at the Royal Surrey County Hospital (Guildford, UK). Five donations were taken, with a minimum of 13 weeks between each donation. The blood was allowed to clot at room temperature, centrifuged, and the serum pipetted into an acid washed container which was then stored frozen at -70 °C ± 10 °C. Each batch of serum collected was added directly into the same bottle. The ‘clean’ serum was mixed thoroughly and then 1.1 mL dispensed into 2 mL plastic screw® cap Nalgene cryovials (Jencons, Leighton Buzzard, UK). Approximately 1000 units of the candidate reference material were produced, which were stored at -70 °C ± 10 °C. keywords: biological material , body fluids , blood , trace elements
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