Shimadzu Europe - AXIMA-TOF2™

The next generation in MALDI CID MS/MS
A TOF-TOF mass spectrometer with high energy MS/MS

Key benefits and features:

• Delivers the highest energy collisions (CID with laboratory energy of 20keV) to unlock more information with higher sensitivity.
• Incorporates novel ion gate technology for high resolution precursor ion selection, simplifying TOF-TOF spectra and data interpretation, even in the presence of complex mixtures
• Opens new horizons in analytical research from peptide characterisation, protein biomarker discovery and lipidomics to polymer characterisation.
• Helps to increase sample throughput and data quality with intelligent results-dependent analysis.
• Ion generation. Using the pioneering technology developed on the AXIMA Curved Field Reflectron platforms, the ion source is designed to maximise ion generation and signal intensity using near-axis laser irradiation.
• Precursor Ion Selection. Once ions are generated they are transmitted through grid-free ion optics (TOF1) to the novel ion gate where ions of interest are selected with high resolution.
• Ion Fragmentation. Following precursor ion selection, ions are transferred to the collision cell for fragmentation. High energy MS/MS is achieved by collision induced dissociation (CID) of selected precursor ions, using helium as the collision gas.
• Ion detection. The selected ions then pass to the gridless curved field reflectron for high resolution mass analysis and detection using an optimised, high performance transient recorder.

The AXIMA-TOF² is supported by a complete suite of software tools designed to simplify and accelerate your workflows.

A diverse range of applications are supported, including fully automated proteomics experiments, LC MALDI analyses, functional genomics, quality control and polymer interrogation.

Proteomics suite

• Designed with the flexibility to adapt to users' workflow: from single sample manual acquisition to fully automated data-dependent peptide mass fingerprinting and MS/MS for protein identification.
• Peptide mass fingerprints are acquired and subjected to an optional integrated Mascot® database search.
• User defined acceptance limits for PMF-based protein identification.
• Data-dependent MS/MS: using the results of the PMF search, MS/MS may be performed on ions that matched to the top ranked protein hit (confirmation MS/MS), in addition to those that were not (investigation MS/MS). Batch searching of these MS/MS spectra is then performed automatically to provide further and higher confidence protein identification.
• Data may be reprocessed and resubmitted for database searching at a later time to provide additional information.

• The AXIMA-TOF²™ provides total support for LC-MALDI based experiments.
• Allows the fully automated acquisition of LC separated samples deposited onto MALDI targets and the subsequent identification of proteins via MS/MS of the peptides detected.
• Automatically provides a provisional intensity map of all sample spots across the target to assess the distribution of peptides and identify the position of the apex of the chromatographic peaks. These are utilised to generate a candidate list and MS/MS data acquired for all discrete peptide ions.
• Exclusion lists are used to remove known contaminants or high abundance peptides.
• All data is subjected to an integrated Mascot® search.
• Low sample consumption allows multiple spectra to be acquired from the same spot increasing the amount of MS/MS data obtained.