Instrument Database:
Shimadzu Europe - Nexera Mikros - Micro-Flow LC-MS solution
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| Year of introduction |
2018 |
| Status |
available |
| Company |
Shimadzu Europe
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| Categories |
Mass spectrometer: LC-MS
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The High Sensitivity You Expect from a Low Flow System with the Ruggedness of HPLC Covering the Complete Range from Micro to Semi-Micro
The Nexera Mikros can be used to detect target components with higher sensitivity than with semi-micro flowrate compatible LCMS systems, which perform measurements within a flowrate range of 100 μL/min to 500 μL/min.
In addition, it features shorter analysis times and is more durable than nano flowrate compatible LCMS systems, which perform measurements within a flowrate range of several 100 μL/min to 1 μL/min. Furthermore, the Nexera Mikros improves operability, and at the same time, enables high-sensitivity analysis with smaller system capacity. This is due to the UF-Link, which enables one-touch connection and disconnection between the LCMS ionization interface and the analytical columns.
Incomparable Microflow Ruggedness
Realize the Benefits of Microflow While Enjoying the Ruggedness and Reliability of HPLC
The Nexera Mikros system delivers the improvements you expect from a low-flow system with the ruggedness of HPLC. A plasma blank was protein precipitated, diluted (3x), and injected 1,500 times consecutively. During the consecutive injections, QC sample (40 ppt of nortriptyline) was injected once every 50 injections of plasma blank. Peak areas and retention times of QC sample are plotted below. With 4.38% RSD area repeatability and 0.25% RSD retention time stability, extremely stable operation was demonstrated, without loss of peak shape common in high-throughput analyses of complex matrices.
Robust and Easy-to-Use Microflow ESI Source
Micro-ESI 8060 + CTO-Mikros
Shimadzu’s Micro-ESI LCMS source has been designed for optimal sensitivity and ease of use. An X-Y adjustable stage and viewing camera allow for easy ESI spray needle adjustment. The column oven is mounted on the LCMS to minimize the volume between the exit of the column and the ESI needle. The Micro-ESI probe also features an ESI spray angle optimized for micro flow rates, and is compatible with LCMS-8045/8050/8060 systems. Mounted to the Micro-ESI source is the CTO-Mikros column oven. Stable column temperature, essential to retention time reproducibility, is ensured and columns up to 150 mm in length can be accommodated. To preserve the optimal source adjustment, the CTO-Mikros features an innovative snap-in column locking system, the UF-Link.
System Configuration Options
Direct Injection System
Ideal for sample volume-limited analyses. This system provides rapid and highly sensitive micro LCMS analysis without sample loss.
Trap and Elute System
Ideal for analyses with larger injection volumes or when some degree of sample cleanup is desirable.
Make-Up Flow System
The addition of a post-column make-up flow pump allows mobile phase modifiers to be added to ensure the highest ionization efficiency in the MS source.
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