Spectrolab Analytical - S10-DB

Innovation and Design
The S10-DB UV Vis Near IR Spectrophotometer employs “state of the art “ optical design to ensure true Double Beam optical photometric performance. This unique spectrophotometer offers unparalleled sensitivity from the far ultra violet to the near Infrared and can be used with an external PC or as a stand alone microprocessor based instrument. A wavelength range of 200 to 1100nm is provided as standard with extensions to 190nm when required. The optical design of the 2600 has no equal in terms of throughput and optical homogeneity and this superb optical performance has allowed us to design a spectrophotometer that can function with very narrow optical beam profiles. This we call Ultra Thin Beam Technology (UTB) and we are able to use this to good effect with any type of sample and especially micro samples. Most other commercial instruments are unable to obtain true micro measurements unless the beam is externally masked. In addition to innovative optics, Supersped incorporates a large number of programming utilities as operating tools that ensure both convenience and ease of use. Some of these Tools are in the form of Methods and Data Bank and provide the analyst with a wealth of information designed specifically to make analysis easier and more universal. Typically the information contained in these data libraries relate to various normal spectra, compounds, derivative spectra, solvents, lambda max, methods of analysis, kinetics and more. These libraries can be updated or reconfigured as required.

UTB Technology
The Supersped optical system employs a special type of holographic concave diffraction grating monochromator allowing remarkable performance throughout the entire wavelength range. When used in conjunction with powerful microelectronics, the result is truly spectacular performance. This is used to advantage with our UTB technology allowing us to provide optical beam widths of less than 0.8mm at full resolution.

Double Optical Beam Technology is used in the 2600 providing unprecedented stability and ease of use. Powerful microprocessors coupled to a unique optical platform combines excellent short and long term drift with low stray light, which is the exact requirement for work at high absorbance. In this the 21st Century, it is absolutely necessary to comply with the requirements of many international standards and regulatory bodies and our designers have taken this into account in all aspects of the Supersped design, including providing a limiting resolution of less than 2nm. And just to make sure that analysis is both fast and accurate, the microprocessor controlled optical system is capable of very fast scan rates and high wavelength accuracy
Spectrum

A choice of scan speeds are provided with options for fast, medium slow and very slow to help with the validation of analytical data. For very high-resolution studies it is possible to measure up the 4th derivative ensuring the exact determination of wavelength maxima and will be found particularly useful when absorbance bands are poorly resolved. Data processing functions include data storage, smoothing, peak pick, derivatives, print, zoom etc. It is also possible to superimpose any number of spectra up to a maximum of 99, a unique facility for the 2600 system.

Photometric
In addition to conventional wavelength scanning, the 2600 also allows specific or incremental wavelength measurements. In this mode up to X10 wavelengths may be pre programmed allowing the system software to perform multi wavelength analysis. Many other features are also provided including auto zero, auto scaling, customised printouts etc. Quantitative Standard calibration graphs may be displayed in direct, linear, quadratic or user defined. It is also possible to display sampled points on stored calibration graphs. In addition up to three specific methods of analysis are possible.

Time Scan
Supersped photometric specifications provide for very low short and long term drift and hardly any system noise. As such it allows the user to obtain unique data in kinetic experiments of all types including reaction kinetics, where absorbance or transmission measurements have to be made as a function of time and sometimes for quite long periods.
Reaction Kinetics

This requires the measurement of the rate of change in absorbance of a sample, normally at constant temperature and Supersped allows the user to monitor such changes with a variety of analytical tools. For example it is possible to measure absorbance with temperature as a function of time using appropriate conversion factors and parameters such as measurement lag time or rate. A programmable multi cell holder is provided for reaction kinetic measurements.
Multi Component Analysis (MCA)

This mode of operation is used to simultaneously determine the concentration of up to ten separate specific components that make up a complex mixture whether it be a pure sample or a mixed standard. MCA dramatically reduces interferences between mixture components.


Software
The standard Supersped software uses a Windows platform for quick and intuitive use. System parameters are normally under complete software control allowing easy programming of any experiment including report or data management. An on line help menu is provided to assist the user along with simple dialogue prompts. Practically unlimited spectra storage is provided along with software features that allow the user to do just about anything that is possible in absorption spectrophotometry
Diagnostics

It is always nice to know for sure that all instrument functions are working correctly and to assist the user we have automated system diagnostics, which provide data relating to power on, deuterium and tungsten lamp output as well as filter position. In addition the system provides for auto lamp alignment wavelength and baseline correction plus a lot more.
Soft Data Banks (SDB)

Supersped simplifies routine operations by providing a remarkable feature called Soft Data Bank. SDB is a file of data defined for a specific analysis and contains normal as well as derivative spectra of a large number of compounds. This data can be utilised as part of a measurement procedure or used as a reference.
Information Bank (IB)

This unique bank contains information relating to the name of a sample, the solvent used in the measurement, wavelength and absorbance values for over 150 compounds, and all of which are linked to the data bank (DB)
Method Bank (MB)

As the name suggests the method bank is used to store information relating to various analytical methods plus any useful references. This can include references concerning complexing agents, pH working range, interferences, formulation procedures as well as metallurgical and environmental analytical data.
Diagnostics

It is always nice to know for sure that all instrument functions are working correctly and to assist the user we have automated system diagnostics, which provide data relating to power on, deuterium and tungsten lamp output as well as filter position. In addition the system provides for auto lamp alignment wavelength and baseline correction plus a lot more.
Soft Data Banks (SDB)

Supersped simplifies routine operations by providing a remarkable feature called Soft Data Bank. SDB is a file of data defined for a specific analysis and contains normal as well as derivative spectra of a large number of compounds. This data can be utilised as part of a measurement procedure or used as a reference.
Information Bank (IB)

This unique bank contains information relating to the name of a sample, the solvent used in the measurement, wavelength and absorbance values for over 150 compounds, and all of which are linked to the data bank (DB)
Method Bank (MB)

As the name suggests the method bank is used to store information relating to various analytical methods plus any useful references. This can include references concerning complexing agents, pH working range, interferences, formulation procedures as well as metallurgical and environmental analytical data.
Water & Beverages

Temperature Controlled Cell Holder
This valuable accessory employs a constant temperature water bath to control the temperature of both the sample and reference cell to between 5 and 900C. Normally used with 10mm rectangular PL cells.
Specular Reflectance and ATR

Both of these sampling methods are available allowing the instrument to be used for complex applications concerning both colour differentiation, film thickness measurements and analysis of highly absorbing materials
Water, Beverages & Soft Drinks Software Programmes

Quite apart from the many thousands of applications that the S10-DB can be used for, we have also developed an optional unique programme for the water, beverages and soft drinks industry. This programme provides default method values for all analytical parameters used making the Supersped the most important spectrophotometer for the drinks and water industry. Although values can be edited to suit given applications when required, standard software includes programmes for water analysis, phosphate assays, beverage colour and sugar colour. As such nearly all of the world’s drinks manufacturers can now automate their analytical procedures with Supersped.
Water analysis

This powerful application programme includes specific operations for the elemental analysis of water using either standards or specific factors for determining Iron ( Fe ) and Manganese ( Mn ). Methods are also included for Chlorine, Phosphates, Sulphates and Chlorides.

GLP & Validation
This feature is now an important part of many analytical procedures providing benchmarking test details and reports. Such tests include

• Noise
• Baseline flatness
• Absorbance stability for 1 hour
• Wavelength accuracy (holmium or D2)
• Photometric accuracy using 1ABS standard
• Photometric accuracy using potassium dichromate
• Stray light test using NO/NO2 & NaL
• Resolution using toluene

e-SOPs
Electronic Standard Operating Procedures (eSOPs) are available and provide information relating to system installation, basic operation, calibration, performance checks, routine maintenance, reporting etc. which sets a basis for DQ,OQ,IQ,PQ. Parameters can be changed to suit individual requirements and can be modified to develop customised e-SOPs. Typical GLP features of the Supersped software include information relating to lamp life or hours used, automated service history, password protection, software validation plus a unique comprehensive service manual.
Autosampler AS 60

Supersped employs an easy to use autosampler with simple operating features that ensure trouble free operation. The AS60 sampler is used under software control via an RS232 interface. Two high precision X&Y translators ensure accurate positional control for both the horizontal axis and the integrated peristaltic pump.
Cell Holders

The Supersped design offers a choice of cell holders to suit specific requirements and includes a 10mm cell holder, a film holder, a long path cell holder able to accommodate 10,20,50 and 100mm rectangular path length cells.

Water & Beverages
Water, Beverages & Soft Drinks Software Programmes

Quite apart from the many thousands of applications that the S10-DB can be used for, we have also developed an optional unique programme for the water, beverages and soft drinks industry. This programme provides default method values for all analytical parameters used making the Supersped the most important spectrophotometer for the drinks and water industry. Although values can be edited to suit given applications when required, standard software includes programmes for water analysis, phosphate assays, beverage colour and sugar colour. As such nearly all of the world’s drinks manufacturers can now automate their analytical procedures with Supersped.
Water analysis

This powerful application programme includes specific operations for the elemental analysis of water using either standards or specific factors for determining Iron ( Fe ) and Manganese ( Mn ). Methods are also included for Chlorine, Phosphates, Sulphates and Chlorides.

Cola & Soft Drinks
Colas, Phosphate Assays and Cola type drinks

This excellent programme will be found to be very useful in determining the phosphoric acid content of syrup or standard beverages. The analytical method uses a molybdate assay to calculate the concentration of phosphoric acid. Data is provided as a function of either sample volume or weight.
Beverage Colour

The beverage industry employs very strict quality controls on all products in terms of colour and Supersped software allows measurement of such colour relative to known standards.

Both RBU and ICUMSA methods may be selected to determine sugar colour

Technical Data
Photometry System:                 Double Beam
Measuring range:                     200-1100nm. (Standard) 190-1100nm (optional)
Monochromator:                      1200 lines/mm concave holographic grating
Spectral Bandwidth:                  <2.0 nm
Wavelength Readability:           0.1 nm increment
Wavelength Accuracy:               ± 0.5 nm
Wavelength Reproducibility:      ± 0.1 nm
Wavelength Scanning Speed:    at sampling interval of 1 nm
Positioning:                             3600 nm/min
Fast:                                      2400 nm/min
Medium:                                 1800 nm/min
Slow:                                      900 nm/min
Very Slow:                               450 nm/min
Sampling interval:
Automatic selection depending on range as follows:
Scanning range Interval
500 nm > ranges =900 nm 1.0 nm
200 nm > range =500 nm 0.5 nm
100 nm > range =200 nm 0.2 nm
10 nm > range =100 nm 0.1 nm
Light Source Selection:           Automatic, according to set wavelengths
Stray Light:                           Less than 0.1 %T at 220 nm.
Photometry Range:                -2.5 to 2.5 Abs
Photometry Accura                 ± 0.005 Abs at 1.0 Abs. with NBS 930 standard filter
Photometry Reproducibility:    ± 0.002 Abs at 1.0 ABS
Auto Zero Function:                Available as standard
Drift:                                    Within ± 0.002 Abs/hr after 2 hours of warm up
Base Line Correction:             Automatic, with built-in memory
Base Line Fitness:                 ± 0.002 Abs excluding spike noise
On line spectrum plotting
Spectrum overlay of up to 99 spectra
Spectrum saving and retrieving facility to & from hard disk.
Spectrum Manipulations including Addition, Subtraction, Multiplication and Division between any two channel spectra Zoom function
Derivative (1st – 4th) Smoothing
Cursor edit function
Multi Component Pure as well as mixed standard samples can be Determination: analysed. (Up to 10 standards can be measured)
Detector:                 Matched pair of Silicon Photo Diodes
Light Source:           50 W, Long life Tungsten-Halogen lamp and Deuterium lamp
Sample compartment:   Fixed cuvette holders for sample and reference
Control: Windows™ based Spectrum™ software
Display: SVGA Colour / Monochrome display
Dimensions: 600*510*215mm
Weight:                        45Kg
Power:                          230V AC 50Hz 200W ±10%