The Thermo Scientific™ Orbitrap Exploris™ 480 mass spectrometer is a Thermo Scientific™ quadrupole-Orbitrap™ mass spectrometer, based on the hardware and instrument control software designs of the next-generation Thermo Scientific™ mass spectrometers.1Built on the guiding principle of ease-of-use and reliable hardware, robust system performance boosts sample throughput. Soundness of data is assured with high-resolution accurate-mass (HRAM) selectivity, high scan speed and best-in-class mass spectral quality, all within a compact footprint to conserve bench space.Simplified operation, smart scheduling and execution of user selected scan types deliver rich, high-confidence sample insights for users of all skill levels in a wide range of applications from small molecules to peptides and intact proteins.
Benefits
- High-performance featuring ultra-high-field Orbitrap mass analyzer technology
- Next-generation software with intuitive tuning, single-click calibration, drag-and-drop method setup, and pre-defined experiments
- Prolonged robustness and improvedserviceably increase uptime
- Hardware and software shared with next-generation Thermo Scientific™mass spectrometers1 streamlines method transfer and familiarization
- Compatible with Thermo Scientific™next generation ion sources including FAIMS Pro interface
- Enhanced peptide quantitation with advanced operating modes
- Thermo Scientific™ BioPharma option for analysis of large biomolecules
Hardware features
Ion source
Thermo Scientific™ OptaMax™ NG ion source
- Adjustable heated electrospray ionization (HESI) probe for ultimate sensitivity
- Additional non-heated low-flow calibrant sprayer for optimal mass and system calibration
- Designed for maximum performance with minimum adjustment
- Enhanced ruggedness and reduced chemical noise with sweep gas
- Flow rates from 1 μL/min to 1000 μL/min; OPTON-30139 (low-flow needle; 1–10 μL/min), OPTON-30694 (high-flow needle; 5–1000 μL/min)Ion opticsThe atmospheric pressure ionization (API) interface consists of:High-capacity ion transfer tube (HCTT)
- Improved sensitivity and desolvation with increased ion flux into the vacuum system
- Vent-free maintenance
Electrodynamic ion funnel (EDIF)
- A radio frequency (RF) device, efficiently captures ions as they leave the HCTT
- Automatic tuning results in broad ion transmission curves with reduced ion losses, increasing sensitivity
Advanced active beam guide (AABG)
Axial field and injection filtering reduce noise by preventingneutrals and high-velocity clusters from entering the quadrupole mass filter using double bent design geometry
Advanced quadrupole technology (AQT)
- Segmented quadrupole mass filter for precursor ion selection with variable precursor isolation width from 0.4 to 1200 u
- MS/MS precursor ion selection with high transmission from m/z 40 to 2500• Prolonged uptime due to patented configuration switching mode
Ion-routing multipole (IRM)
- Robust ion trapping for MS scans and higher energy collisional dissociation (HCD)
- Selection of absolute collision energy (CE) or normalizedcollision energy (NCE) for precursor fragmentation
- Nitrogen collision gas
Software features
Data System
- High-performance PC with Intel® microprocessor
- High-resolution LED color monitor
- Microsoft® Windows® 10 Enterprise (Long Term Service version) operating system
- High-speed real-time data acquisition and instrument control
- Automatic calibration of all ion transfer and analysis parameters via instrument control software
Thermo Scientific™ Xcalibur™ software
- Xcalibur software is the control software for the next-generation Thermo Scientific mass spectrometer portfolio
- Accelerates familiarization and reduces training needs
Thermo Scientific™ Orbitrap Exploris™ instrument control software
- Tune application for instrumental mass and system calibrations and checks, diagnostics, and manual data acquisition
- Method Editor with a comprehensive application-specific template library, method setup supported by tooltips, and a and drag-and-drop user interface to facilitate method development
Optional software
Thermo Scientific™ Proteome Discoverer™software
Simplifies a wide range of proteomics workflows, from protein and peptide identification to PTM analysis to isobaric mass tagging, and SILAC and label-free quantitation
Thermo Scientific™ BioPharma Finder™ software
Provides complete protein characterization, including intactprotein mass analysis, top- and middle-down analysis, peptide mapping or multi-attribute method (MAM) workflows
Thermo Scientific™ ProSightPC™ software
Stand-alone software for analyzing top-down, middle-down, and bottom-up data
Thermo Scientific™ Compound Discoverer™software
Streamlines small molecule unknown identification, determination of real differences between samples, and elucidation of biological pathways with an integrated suite of data analysis tools
Operation modes
Resolution settings
Ranging from 7500 to 480,000 at m/z 200
TurboTMT intelligent data acquisition mode
Novel processing mode powered by Phased Spectrum Deconvolution Method (ΦSDM) specifically designed to improve the acquisition rate of TMT experiments
Advanced peak determination (APD)
Precursor annotation algorithm for improved charge state assignment to increase the number of precursors available for data-dependent analysis
Application mode
Sets optimal default instrument parameters and manages application-specific system templates for easy method development and execution.
Available modes are:
- Small molecule
- Peptide
- Intact protein (included with BioPharma option)
Scan functions
- Full scan
- SIM scan: Selected Ion Monitoring
- in DDA* manner (discovery)
- in targeted SIM manner based upon a sample-dependent, user-defined mass list– with Targeted Mass Filter applied (confirmation)
- AIF scan: All Ion Fragmentation
- MS2 scan by Data Dependent Acquisition (DDA) following a master scan (e.g., a Full scan):
- in TopN or Top Speed manner (discovery)
- with Targeted Mass Filter applied (confirmation)
- by combining discovery and conformation manner
- MS2 scan: targeted MS2 (Parallel Reaction Monitoring), based upon a sample-dependent, user-defined mass list
- MS2 scan by Data Independent Acquisition (DIA**)
- w/ or w/o interspersed, data independent Full scan (e.g.)
- with isolation widths settable per experiments
* a Data Dependent Acquisition (DDA) is an acquisition manner in which a decision for a scan execution is based upon certain criteria met in a fore-running master scan
** a Data Independent Acquisition (DIA) is an acquisition manner in which the scan execution is independent from any criteria met in a fore-running scan; there is no need for a fore-running scan.
