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The establishment of EVISA is funded by the EU through the Fifth Framework Programme (G7RT- CT- 2002- 05112).

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gradient elution

An HPLC procedure where the composition of the mobile phase changes during the separation; opposite of isocratic elution where the mobile phase composition remains constant throughout the entire chromatogram. A typical example would be where the mobile phase starts out as water, and methanol is added continuously so that the percentage of methanol in the mobile phase increases from zero to 100% during the chromatographic run.

The term "gradient elution" was found in the following pages:


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