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Bromine Speciation in Human Serum


Bromine is an essential trace element for animals and humans. Bromine acts as a sedative-hypnotic and it has important functions in the formation of collagen IV and in the activation of α-amylase in saliva. Bromine determination at low concentration is a challenge and just a few techniques may provide accurate results. Inductively coupled plasma mass spectrometry (ICP-MS) is one of the most sensitive technique for ultra-trace elemental determination, but the determination of the halogens is not that favourable because of their high ionization potentials and interference effects related to the carbon containing matrix. The blood plasma level of Br− is more than 1,000-fold lower than that of chloride anion (Cl−). By using SF-ICP-MS, Rodushkin et al. (2001) [PE84852] have found 3.4-8.4 mg/L in whole blood and about one third in serum.

The new study:
The authors combined different columns coupled with ICPMS to reveal the natural state of bromine in human serum. By using size-exclusion chromatography coupled to ICP-MS (SEC-ICP-MS) the authors found three bromine species in human serum. The most abundant species was identified as Br− by anion exchange chromatography–inductively coupled plasma mass spectrometry (IC–ICP-MS). Results for the determination of Br− and total bromine in human serum revealed that Br− is the major form of bromine in human serum, with a ratio ranging from 57.3 to 68.8%.

The cited study:

Deye Liu, Geng Chen, Zongli Huo, Hong Liu, Wenling Ji, Hualiang Liu, A Study of Bromine Speciation in Human Serum and Ambroxol Determination in Rat Plasma by Liquid Chromatography–Inductively Coupled Plasma Mass Spectrometry, Chromatographia, 82/6 (2019) 927–934. DOI: 10.1007/s10337-019-03730-z

Related studies (newest first)

Catarine G.L. Canellas, Silvia M.F. Carvalho, Roberta G. Leitão, Alfredo V.B. Bellido, Marcelino J. Anjos, Ricardo T. Lopes, Multielement analysis in serum of healthy population of the metropolitan region of Rio de Janeiro in Brazil by SRTXRF, X-Ray Spectrom., 45 (2016) 14–18. DOI: 10.1002/xrs.2631

P. Avino, G. Capannesi, M. Manigrasso, S. Sabbioni, A. Rosada, Element assessment in whole blood,serum and urine of three healthy sub-populations by INAA, Microchem. J., 99/2 (2011) 548-555. DOI: 10.1016/j.microc.2011.07.008

L.C. Oliveira, C.B. Zamboni, S. Metairon, Reference values in blood from inhabitants of Brazil: Br,Cl, K and Na determination using NAA, J. Radioanal. Nucl. Chem., 282/1 (2009) 95-97. DOI: 10.1007/s10967-009-0276-3

Kai-en Wang, Shiuh-Jen Jiang, Determination of Iodine and Bromine Compounds by Ion Chromatography/Dynamic Reaction Cell Inductively Coupled Plasma Mass Spectrometry, Anal. Sci., 24 (2008) 509-514. DOI: 10.2116/analsci.24.509

L.C. Oliveira, C.B. Zamboni, J. Mesa, Quantitative estimation of Br, Cl, K and Na in sample blood by NAA, J. Radioanal. Nucl. Chem., 269/3 (2006) 541-545. DOI: 10.1007/s10967-006-0262-y

Gordon W. Gribble, Natural Organohalogens: A New Frontier for Medicinal Agents?, J. Chem. Educ., 81/10 (2004) 1441-1449. DOI: 10.1021/ed081p1441

I. Yanagisawa, S. Torii, A bromine compound existing in blood, Tohoku J. Exp. Med., 196/3 (2002) 111-121. DOI: 10.1620/tjem.196.111

I. Rodushkin, F. Ödman, S. Branth, Multielement analysis of whole blood by high resolution inductively coupled plasma mass spectrometry, Fresenius J. Anal. Chem., 364/4 (1999) 338-346. DOI: 10.1007/s002160051346

H.S. Olszowy, J. Rossiter, J. Hegarty, P. Geohagan, M. Haswell-Elkins, Background levels of bromide in human blood, J. Anal. Toxicol., 22/3 (1998) 225-230. DOI: 10.1093/jat/22.3.225

Valeri V. Salov, Jun Yoshinaga, Yasuyuki Shibata, Masatoshi Morita, Determination of Inorganic Halogen Species by Liquid Chromatography with Inductively Coupled Argon Plasma Mass Spectrometry, Anal. Chem., 64 (1992) 2425-2428. DOI: 10.1021/ac00044a021

F.M. Corrigan, E.R. Skinner, F. MacIntyre, G. Ijomah, J. Holliday, D.F. Horrobin, N.I. Ward, Plasma bromine concentrations and lipid profiles, Clin. Chim. Acta, 204/1–3 (1991) 301-304. DOI: 10.1016/0009-8981(91)90240-D

C. Vandecasteele , H. Vanhoe, R. Dams, J. Versieck, Determination of bromine in human serum by inductively coupled plasma-mass spectrometry, Anal. Lett., 23/10 (1990) 1827-1841. DOI: 10.1080/00032719008052530
R.E. Cuenca, W.J. Pories, J. Bray, Bromine levels in human serum, urine and hair, Biol. Trace. Eelem. Res., 16 (1988) 151-154. DOI: 10.1007/bf02797099
Z.B. Alfassi, N. Lavi, Determination of bromine in blood serum by epithermal neutron activation analysis, Anal. Chem., 55/4 (1983) 796-797. DOI: 10.1021/ac00255a049

I. Yanagisawa, H. Yoshikawa, A bromine compound isolated from human cerebrospinal fluid, Biochim. Biophys. Acta, 329/2 (1973) 283-294. DOI: 10.1016/0304-4165(73)90293-6

last time modified: November 27, 2023


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