A group of Chinese researchers used Liquid Chromatography–Inductively Coupled Plasma Mass Spectrometry for the speciation analysis of bromine in human serum. Bromide was identified as the most prominent species.
Background:Bromine is an essential trace element for animals and humans. Bromine acts as a sedative-hypnotic, and it has important functions in the formation of collagen IV and in the activation of α-amylase in saliva. Bromine determination at low concentration is a challenge, and just a few techniques may provide accurate results. Inductively coupled plasma mass spectrometry (ICP-MS) is one of the most sensitive technique for ultra-trace elemental determination, but the determination of the halogens is not that favourable because of their high ionization potentials and interference effects related to the carbon containing matrix. The blood plasma level of Br− is more than 1,000-fold lower than that of chloride anion (Cl−). By using SF-ICP-MS, Rodushkin et al. (2001) [PE84852] have found 3.4-8.4 mg/L in whole blood and about one third in serum.
The new study:The authors combined different columns coupled with ICP-MS to reveal the natural state of bromine in human serum. By using size-exclusion chromatography coupled to ICP-MS (SEC-ICP-MS) the authors found three bromine species in human serum. The most abundant species was identified as Br− by anion exchange chromatography–inductively coupled plasma mass spectrometry (IC–ICP-MS). Results for the determination of Br− and total bromine in human serum revealed that Br− is the major form of bromine in human serum, with a ratio ranging from 57.3 to 68.8%.
The cited study:
Deye Liu, Geng Chen, Zongli Huo, Hong Liu, Wenling Ji, Hualiang Liu,
A Study of Bromine Speciation in Human Serum and Ambroxol Determination in Rat Plasma by Liquid Chromatography–Inductively Coupled Plasma Mass Spectrometry, Chromatographia, 82/6 (2019) 927–934.
DOI: 10.1007/s10337-019-03730-z
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