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Instrument Database:
Bruker Daltonics - ultraflex II & ultraflex II TOF/TOF
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| Year of introduction |
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| Status |
historical ( out of sale ) |
| Company |
Bruker Daltonics
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| Categories |
Mass spectrometer: MALDI
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Features the latest advancements in the sensitivity, resolution and mass accuracy of MALDI-TOF and TOF/TOF technology for high-success expression proteomics and advanced biomarker discovery studies.
The latest advancements in MALDI-TOF and TOF/TOF MS
- The ultraflex II incorporates next-generation MALDI-TOF and TOF/TOF technology, including
- Greatly increased sensitivity by an order of magnitude, delivered by advanced LIFT technology and ion optics, as well as enhanced TOF/TOF resolution
- High mass resolution over a broad mass range, delivered by the proprietary panoramic PAN technology
- Unique T3-sequencing capabilities, allowing top-down sequence analysis on many intact proteins with ease
- High-throughput analysis and sensitive detection of labile protein modifications delivered by tunable laser speed from 1-200Hz
Uncompromised TOF-MS performance
The ultraflex II uses our patented gridless ion source and reflectron design, providing unsurpassed sensitivity in the standard MALDI-TOF MS mode. The "TOF only" configuration is field upgradeable to full MALDI-TOF/TOF capability. Superior MS/MS data - at the speed of MALDI-TOF! Measure MS/MS spectra with rapid precursor ion selection and our proprietary LIFT technology. Full MS/MS data sets are acquired in just seconds!
Consistent automation
With full microtiterplate compatibility the ultraflex II seamlessly interfaces with the biochemical lab. Our patented AnchorChip MALDI target technology provides homogeneous, exactly-positioned samples for robust and fast automation, as well as a sensitivity boost by an order of magnitude.
smartbeam™ technology
Experience uncompromised performance based on a modified All-Solid-State Laser System. With smartbeam, application possibilities of a solid-state laser are dramatically expanded:
maintain uncompromised dried-droplet HCCA performance
achieve highest sensitivity from thin layer preparations (enables on-target sample purification)
maximize linear instrument performance for large protein applications
increase the variety of useful matrices (e. g. DHB, SA)
gain perfect access to MALDI-Imaging with highest repetition rates
Expanding possibilities in all applications – from thin layer robotics preparations to large proteins and CLINPROT applications, smartbeam offers significant enhancements in sensitivity and mass resolution compared to a classical 200 Hz Nd:YAG laser.
smartbeam combines the advantages of both, the known nitrogen laser performance and the highest reliability of all-solid-state lasers.
Next-generation AnchorChip targets
The novel Prespotted AnchorChip MALDI targets in industry-standard microtitre plate (MTP) format provide highest sensitivity, user-friendliness and reliability without the risk of any cross-contamination that is inherent to reusable targets.
Integrated software environment
The ultraflex II operates within the powerful Compass software environment. Compass offers a seamless link with the most comprehensive proteomics software suite in the industry, including the ProteinScape database for proteomics project management.
Applications
High-success Protein Identification from 2D gels
The ultraflex II is part of the PROTEINEER system for peptide mass fingerprinting, peptide fragment analysis and post-translational modifications (PTM) analysis. The ultraflex II TOF/TOF enables high-throughput protein identification by MALDI-TOF peptide mass fingerprinting, immediately followed by more detailed protein characterization using MALDI-TOF/TOF tandem mass spectrometry (MS/MS) on the same prepared sample. Comprehensive MS/MS information is available from minute sample amounts within a few seconds.
De novo sequencing
The ultraflex TOF/TOF provides two MS/MS methods for de-novo sequencing:
1. For highest specificity, LID-LIFT TOF/TOF MS is the method of choice, providing clear spectra with readily available i-, a-, b- and y-ions.
2. For in-depth analysis of selected peptides, high-energy CID (collision-induced decomposition) is available as well.
Protein Identification and Quantitation by LC-MALDI
The PROTEINEER-LC solution package provides the unique, intelligent WARP-LC workflow for LC-MALDI experiments. Low abundant proteins can be identified and characterized at great detail using MALDI-TOF/ TOF. This workflow can be further enhanced by a combination with LC-ESI-MS/MS data. The resulting LC-ESI-MALDI-MS/MS workflow utilizes complementary information from two ionization techniques in a single experiment, providing a new level of insight. Quantitative expression proteomics experiments with various isotope labeling techniques including ICAT™* can be realized as well.
*ICAT is a trademark of the University of Washington.
T3 Sequencing
The unique T3-sequencing capability allows top-down sequence analysis on many intact proteins.
Advanced Biomarker Discovery
The ultraflex II is fully integrated into the CLINPROT solution for high-resolution peptide and protein biomarker discovery, identification and validation.
MALDI Imaging
An innovation in quantitative proteomics is the two-dimensional peptide imaging of cells or tissue extracts called MALDI Imaging. This technology is fully supported by the new flexImaging 1.0 software.
Technical Data
MALDI source
- Long lifetime nitrogen (N2) laser as standard
- Optional smartbeam laser, repetition rate tunable from 1 to 200 Hz
TOF and TOF/TOF Performance
- Higher sequence coverage, search scores and success rate of protein ID through:
- Proprietary PAN™ (panoramic mass range focussing) technology
- High-Precision Calibration (HPC™) for highest mass accuracy
- Advanced AnchorChip™ sample preparation technology
- Ultimate MS and MS/MS sensitivity
- Unique T3 sequencing capability for top-down protein sequence analysis
Automation
scoutMTP™/AnchorChip MALDI targets in 384 and 1536 microtiter plate format
Sample target identification via transponder or optional barcode reader
Compass™ software suite- flexControl™ module for quick and easy instrument control
- Fuzzy-logic based AutoXecute™ engine for on-the-fly optimization of spectral quality
- flexAnalysis module for automated and interactive data analysis, including integration with BioTools™, ProteinScape™, ClinProTools™, GenoTools™, and search engines
- Optional LC-MALDI (WARP-LC™) and MALDI Imaging (flexImaging™) software modules
- Optional Compass Security Pack™ for work in regulated environments (21CFR part 11 compliance)
Support features- Remote on-line service capability via WebEx Internet sessions
- Extended self diagnostics
- Remote on-line service capability via WebEx Internet sessions
- Supplementary IQ/OQ/PV procedures available
System solutions- The ultraflex II is fully integrated with Bruker Daltonics’ system solution
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