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Certified reference materials for mercury in marine animal tissues


Monomethylmercury compounds are of great concern, as these highly toxic compounds are formed biotically by microorganisms in sediment and algae in sea water and/or abiotically. Once it is formed, methylmercury is biopaccumulöated and biomagnified in the aquatic food chains, thus resulting in exposure levels among fish-eating populations, often at levels exceeding what is regarded as a safe level.

It is therefore of primary interest to have samples from the animal species within the food chain at different trophic level.


The main issues to be controlled during the analysis of biological tissues for the speciation analysis of mercury are:
  • quantitativeness of extraction of the species from the tissue
  • absence of artificial formation of methylmercury during the analytical procedure
  • control of contamination mainly for inorganic mercury
  • avoidance of ion-exchange and adsorption problems on GC coilumn packing materials
  • control of the derivatization yield
CRMs play a major role in the development and validation of methods and for quality control. The following certified reference materials (CRMs) are available for the speciation of mercury species in marine animal tissues:
 
Supplier  Name           
Material
Certificate
BCR CRM 422
 Cod fish muscle
BCR CRM 463
 Tuna fish musle
 Total mercury, methylmercury
BCR CRM 464
 Tuna fish muscle
 Total mercury, methylmercury
IAEA
 IAEA 140 
Marine algae homogenate
IAEA IAEA 407
 Sea fish tissue
 (Ag),Al, As, Br, Ca, Cd, Co, Cr, Cu, Fe, Hg, MeHg, K, Li, Mg, Mn, Na, Ni, Pb, Rb, Sb, Se, Sr, V, Zn
IAEA IAEA 346
 Tuna
Hg, MeHg, under preparation
NIST
 SRM 1566 b
 Oyster tissue
 Ca, Cl, Mg, K, Na, S, Al, As, Cd, Co, Cu, Fe, Pb, Mn, Hg, MeHg, Ni, Rb, Se, Ag, Th, V, Zn
NIST SRM 1974 B
 Mussel tissue
 Hg, MeHg, Organics
NIST SRM 2976
 Mussel tissue
 As, Cd, Cu, Fe, Pb, Se, Zn, Total Hg, MeHg
NIST SRM 2977
 Mussel tissue
 Cd, Cu, Pb, Mn, Ni, Sr, PAHs, PCBs, MeHg
NIST SRM 1946
 Freshwater fish
NRCC
 		DORM-2
 		Dogfish muscle
 		Al, As, Cd, Co, Cr, Cu, Fe, Pb, Mn, Hg, Ni, Se, Ag, Tl, Sn, Zn, MeHg
NRCC DOLT-3
 		Dogfish liver
 		As, Cd, Cu, Fe, Pb, Hg, Ni, Se, Ag, (MeHg)
NRCC LUTS-1
 Non-defatted lobster tissue
 As, Cd, Ca, Cr, Co, Cu, Fe, Pb, Mg, Mn, Ni, K, Se, Ag, Sr, Zn, [Hg], [MeHg]
NRCC TORT-2
 Lobster tissue
 As, Cd, Co, Cr, Cu, Fe, Pb, Mn, Hg, Mo, Ni, Se, Sr, Sn, V, Zn, MeHg

* () =information value only, []= original certification no longer valid
NB:! click on the suppliers name to reach his website, click on the material name for a link to the report, click on the certified species for a link to the certificate


last time modified: June 15,2020


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